Kawasaki C, Okamura S, Omori F, Shimoda K, Hayashi S, Kondo S, Yamada M, Niho Y
First Department of Internal Medicine, Kyushu University, Fukuoka, Japan.
Exp Hematol. 1990 Nov;18(10):1090-3.
We present the first report of a T-helper cell line, HUT 102, constitutively producing both granulocyte-macrophage colony-stimulating factor (GM-CSF) and monocytic colony-stimulating factor (M-CSF), as detected by sensitive enzyme-linked immunosorbent assays and Northern blot analysis. However, neither amplification nor structural change of the GM-CSF and M-CSF genes was detected by Southern blot analysis. In the case of HUT 102, in which human T-lymphotropic leukemia virus type I (HTLV-I) is integrated, the viral protein, which acts as a trans-acting transcriptional activator, may induce the production of both GM-CSF and M-CSF.
我们首次报告了一种T辅助细胞系HUT 102，通过灵敏的酶联免疫吸附测定和Northern印迹分析检测到，该细胞系组成性地产生粒细胞-巨噬细胞集落刺激因子（GM-CSF）和单核细胞集落刺激因子（M-CSF）。然而，Southern印迹分析未检测到GM-CSF和M-CSF基因的扩增或结构变化。在整合了I型人类嗜T淋巴细胞病毒（HTLV-I）的HUT 102细胞系中，作为反式作用转录激活因子的病毒蛋白可能诱导GM-CSF和M-CSF的产生。
