Parker S J, Inchley C J
Department of Zoology, University of Edinburgh, Scotland.
Exp Parasitol. 1990 Oct;71(3):249-58. doi: 10.1016/0014-4894(90)90029-c.
The immune response to a primary infection of Heligmosomoides polygyrus (Nematospiroides dubius) was studied by flow cytometry in three strains of mice, BALB/c, CBA, and NIH. The chief feature of the response was a pronounced increase, during the first week, in the proportion of B lymphocytes in the mesenteric lymph nodes. All three strains also showed an increase in lymph node cellularity, although this was delayed in NIH and CBA mice. Total B cell numbers thus also increased, particularly in the BALB/c and NIH strains but only in the latter was this response maintained throughout the 4-week study. Although the early changes in B cell frequency were similar in all three strains, B cell responses were greatest in BALB/c mice, and most prolonged in NIH, when they persisted into the adult phase of the infection. These features distinguished them from CBA mice, and could be associated with known variations in resistance to challenge infections. An increase in T cell numbers was delayed in comparison with the changes in the B cell population, and the ratio of 'helper' to 'suppressor/cytotoxic' T cells remained more or less constant in all three strains. There was thus no evidence for an increase in the frequency of suppressor T cells in any strain of mouse.
通过流式细胞术研究了BALB/c、CBA和NIH这三种品系小鼠对多枝赫利格螺线虫（双盘细颈线虫）初次感染的免疫反应。免疫反应的主要特征是，在第一周内，肠系膜淋巴结中B淋巴细胞的比例显著增加。所有三个品系的淋巴结细胞数量也都有所增加，不过在NIH和CBA小鼠中这种增加出现延迟。因此，总的B细胞数量也增加了，特别是在BALB/c和NIH品系中，但只有在NIH品系中，这种反应在整个4周的研究过程中一直持续。尽管所有三个品系中B细胞频率的早期变化相似，但BALB/c小鼠的B细胞反应最为强烈，而NIH品系的反应持续时间最长，一直持续到感染的成年阶段。这些特征将它们与CBA小鼠区分开来，并且可能与已知的对攻击感染的抵抗力差异有关。与B细胞群体的变化相比，T细胞数量的增加出现延迟，并且在所有三个品系中，“辅助性”T细胞与“抑制性/细胞毒性”T细胞的比例或多或少保持恒定。因此，没有证据表明任何品系小鼠中抑制性T细胞的频率增加。
