Parker S J, Inchley C J
Department of Zoology, University of Edinburgh, Scotland, UK.
J Helminthol. 1990 Mar;64(1):35-45. doi: 10.1017/s0022149x00011858.
Responses to parasite antigens were studied in three strains of mice, BALB/c, CBA and NIH, during the initial phases of a primary infection with the intestinal nematode Heligmosomoides polygyrus. Changes in the rate of in vivo cell division were analysed in mesenteric lymph nodes and spleens during the phases of larval maturation and adult establishment, and related to changes in organ size and cellularity. The nature of the proliferating cell populations was also investigated by flow cytometry, carried out on cell suspensions prepared at the time when larval development was complete. The variation in the ability of the strains of mice to become resistant to a challenge infection was manifest as only slight differences in their initial responses to infection. All three strains showed an increase in 125I-iododeoxyuridine incorporation in their mesenteric lymph nodes and spleen, and an increase in B cell frequency over that of T cells in the draining lymph nodes. Although lymph node weight in NIH mice continued to rise over a 4 week period, the majority of responses measured were short lived, peaking 10 to 14 days after infection. The low responder status of CBA mice was thus reflected in a transient and relatively small enlargement of lymphoid tissues, but their early proliferative responses to antigen were similar in scale to those of responder strains.
在肠道线虫多育赫氏线虫初次感染的初始阶段，研究了BALB/c、CBA和NIH这三种品系小鼠对寄生虫抗原的反应。在幼虫成熟和成虫定居阶段，分析了肠系膜淋巴结和脾脏中体内细胞分裂速率的变化，并将其与器官大小和细胞数量的变化相关联。还通过流式细胞术研究了增殖细胞群体的性质，该分析是在幼虫发育完成时制备的细胞悬液上进行的。小鼠品系对攻击感染产生抗性的能力差异，仅表现为它们对感染的初始反应存在细微差异。所有三个品系的肠系膜淋巴结和脾脏中125I-碘脱氧尿苷掺入量均增加，引流淋巴结中B细胞频率相对于T细胞增加。尽管NIH小鼠的淋巴结重量在4周内持续增加，但所测量的大多数反应都是短暂的，在感染后10至14天达到峰值。因此，CBA小鼠作为低反应品系的状态表现为淋巴组织短暂且相对较小的肿大，但其对抗原的早期增殖反应在规模上与反应品系相似。
