Sell T W, Eckels D D
Immunogenetics Research Section, Blood Center of Southeastern Wisconsin, Milwaukee 53233.
Hum Immunol. 1990 Sep;29(1):23-30. doi: 10.1016/0198-8859(90)90066-x.
Combinations of peripheral blood lymphocytes, matched or mismatched for HLA-DP, were analyzed in primary one-way mixed lymphocyte culture experiments. Proliferative responses as correlated with tritiated thymidine uptake were assessed over a kinetic range of 5-15 days. A proliferative response was observed between DP-mismatched combinations, whereas combinations matched for DP and all other HLA alloantigens did not elicit significant proliferation. Optimal responses were observed 9 days after the combination of 1 x 10(5) responder and stimulator cells. Responses were blocked by anti-DP monoclonal antibodies. These studies demonstrate the complexity of the primary mixed lymphocyte culture system and suggest that DP alloantigens should be considered when anomalous responses are obtained.
在初次单向混合淋巴细胞培养实验中，对HLA - DP匹配或不匹配的外周血淋巴细胞组合进行了分析。通过氚标记胸腺嘧啶核苷摄取来评估增殖反应，该反应在5 - 15天的动力学范围内进行评估。在DP不匹配的组合之间观察到增殖反应，而DP以及所有其他HLA同种抗原均匹配的组合未引发显著增殖。在1×10⁵反应细胞和刺激细胞组合后9天观察到最佳反应。反应被抗DP单克隆抗体阻断。这些研究证明了初次混合淋巴细胞培养系统的复杂性，并表明当获得异常反应时应考虑DP同种抗原。
