Schroeijers W E, de Koster H S, van Rood J J, Termijtelen A
Department of Immunohematology, Leiden University Hospital, The Netherlands.
Tissue Antigens. 1988 Sep;32(3):145-9. doi: 10.1111/j.1399-0039.1988.tb01650.x.
To study the stimulatory capacity of HLA-DR beta III in the mixed lymphocyte culture (MLC) assay, an MLC matrix between 13 Dw18 homozygous typing cells (HTCs) was analysed. Six of these HTCs were positive for the HLA-DR beta III allele LB-Q1 as defined by T cell clones. Seven HTCs were positive for LB-Q4. The MLC responses between Dw18 HTCs, matched for LB-Q, were significantly lower than the responses between the mismatched combinations. Considering the fact that HLA-DP can induce proliferation in MLC, we then analysed the DP matched and mismatched combinations separately. The influence of HLA-DR beta III mismatches was in our experiments comparable to the influence of mismatches for HLA-DP. Surprisingly, 15 out of 22 DR beta III and DP matched combinations still showed positive MLC reactions.
为研究HLA-DRβIII在混合淋巴细胞培养（MLC）试验中的刺激能力，分析了13个Dw18纯合分型细胞（HTC）之间的MLC反应格局。其中6个HTC对T细胞克隆所定义的HLA-DRβIII等位基因LB-Q1呈阳性。7个HTC对LB-Q4呈阳性。对于LB-Q相匹配的Dw18 HTC之间的MLC反应，显著低于不匹配组合之间的反应。鉴于HLA-DP可在MLC中诱导增殖，我们随后分别分析了DP匹配和不匹配的组合。在我们的实验中，HLA-DRβIII不匹配的影响与HLA-DP不匹配的影响相当。令人惊讶的是，22个DRβIII与DP匹配的组合中有15个仍显示出阳性MLC反应。
