水稻旗叶叶舌长度的数量性状位点定位及与 ZmLG1 基因的共线性分析。

Quantitative trait loci mapping of flag-leaf ligule length in rice and alignment with ZmLG1 gene.

机构信息

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.

出版信息

J Integr Plant Biol. 2009 Apr;51(4):360-6. doi: 10.1111/j.1744-7909.2008.00803.x.

DOI:10.1111/j.1744-7909.2008.00803.x

Abstract

A doubled haploid (DH) population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid ‘CJ06’/‘TN1’, was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci (QTL) mapping using the DH population. Five QTLs (qLL-2, qLL-4, qLL-6, qLL-10 and qLL-12) controlling the ligule length (LL) were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL-4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.

摘要

本研究利用由籼粳杂交品种‘CJ06’/‘TN1’花药培养获得的 120 个株系组成的加倍单倍体（DH）群体，对旗叶叶舌长度进行数量性状位点（QTL）作图分析。在第 2、4、6、10 和 12 染色体上共检测到控制叶舌长度（LL）的 5 个 QTL（qLL-2、qLL-4、qLL-6、qLL-10 和 qLL-12），解释方差分别为 11.4%、13.6%、27.8%、22.1%和 11.0%。利用玉米 MaizeGDB 中的四个已知基因 ZmGL1、ZmGL2、ZmGL3 和 ZmGL4，在水稻中对其同源基因进行了比对，并通过计算机映射将其整合到现有的简单重复序列连锁图谱中。一个编码居间区结合蛋白的 squamosa 启动子结合蛋白基因 ZmLG1 同源物 OsLG1 位于标记 RM255 和 RM280 之间，正好与第 4 染色体长臂上 qLL-4 的区间相同。该结果有助于叶舌分子机制的剖析和谷物进化的研究。