J Proteome Res. 2011 May 6;10(5):2684-6. doi: 10.1021/pr2001447. Epub 2011 Apr 6.
The "invisible" proteome of a Cola drink, stated to be produced with a kola nut extract, has been investigated via capture with combinatorial peptide ligand libraries (CPLL). Indeed, a few proteins in the M(r) 15-20 kDa range could be identified by treating large beverage volumes (1 L) and performing the capture with CPLLs at very acidic pH values (pH 2.2) under conditions mimicking reverse-phase adsorption. Ascertaining the presence of proteins deriving from plant extracts has confirmed the genuineness of such beverage and suggests the possibility of certifying whether soft drinks present on the market are indeed made with vegetable extracts or only with artificial chemical flavoring.
通过组合肽配体文库（CPLL）捕获，研究了据称用可乐果提取物生产的可乐饮料的“隐形”蛋白质组。实际上，通过处理大体积饮料（1 L）并在模拟反相吸附的条件下在非常低的 pH 值（pH 2.2）下用 CPLL 进行捕获，可以鉴定出一些在 15-20 kDa 范围内的蛋白质。确定源自植物提取物的蛋白质的存在证实了这种饮料的真实性，并表明有可能证明市场上的软饮料是否确实是用植物提取物制成的，还是仅用人工化学调味剂制成的。
