Hickman C J, Crim J A, Mostowski H S, Siegel J P
Laboratory of Cellular Immunology, Food and Drug Administration, Bethesda, MD 20892.
J Immunol. 1990 Oct 15;145(8):2415-20.
The effects of IL-7 on the generation of human CTL in alloantigen-, virus-, and lectin-stimulated systems were examined. Addition of IL-7 at the onset of cultures resulted in marked (up to 80-fold) augmentation of cytotoxicity accompanied by smaller (1.5- to 4-fold) increases in total lymphocyte number. Studies of CTL development in purified lectin-stimulated CD8+ T cell populations demonstrated that IL-7 could act directly on the CD8+ lymphocyte subset to augment cytotoxicity. In MLC, the IL-7-induced enhancement of cytotoxicity was found to be mediated primarily by the CD8+ subpopulation of lymphocytes. Late addition of IL-7 (day 5 of 7) resulted in an increase in cytolytic activity that was associated with little or no increase in total or activated CD8+ lymphocyte number indicating that IL-7 may act as a differentiation factor for human CTL. A role for endogenous IL-7 in CTL development was suggested by the observation that addition of neutralizing antiserum to IL-7 to MLC at initiation (or 5 days thereafter) resulted in decreased levels of cytotoxicity. These results indicate that IL-7 can exert major up-regulatory effects on human CTL development and suggest that these effects are both proliferative and differentiative.
研究了白细胞介素-7（IL-7）在同种异体抗原、病毒和凝集素刺激系统中对人细胞毒性T淋巴细胞（CTL）生成的影响。在培养开始时添加IL-7导致细胞毒性显著增强（高达80倍），同时总淋巴细胞数量有较小幅度的增加（1.5至4倍）。对纯化的凝集素刺激的CD8 + T细胞群体中CTL发育的研究表明，IL-7可直接作用于CD8 +淋巴细胞亚群以增强细胞毒性。在混合淋巴细胞培养（MLC）中，发现IL-7诱导的细胞毒性增强主要由淋巴细胞的CD8 +亚群介导。后期添加IL-7（第7天的第5天）导致溶细胞活性增加，而总CD8 +淋巴细胞或活化的CD8 +淋巴细胞数量几乎没有增加或没有增加，这表明IL-7可能作为人CTL的分化因子。通过在开始时（或此后5天）向MLC中添加针对IL-7的中和抗血清导致细胞毒性水平降低的观察结果，提示内源性IL-7在CTL发育中起作用。这些结果表明，IL-7可对人CTL发育发挥主要的上调作用，并提示这些作用既是增殖性的也是分化性的。
