Department of Military Hygiene, Second Military Medical University, Shanghai, China.
Nutrition. 2011 Oct;27(10):1048-52. doi: 10.1016/j.nut.2010.11.007. Epub 2011 Mar 30.
We investigated the influence of iron supplementation on brain oxidative stress and antioxidase activity in psychologically stressed rats.
Rats were maintained on diets with different iron doses for 1 wk, and all other constituents of the diet were equated exactly according to the AIN-93-G diet. At the end of the experimental period, rats were sacrificed and brains were collected. To evaluate the effect of iron consumption, serum iron, apparent iron absorption, levels of iron concentration, lipid peroxidation, reduced glutathione, and superoxide dismutase activities of brains were measured.
Iron overload significantly elevated the level of iron content and malonaldehyde in rat brain, especially in the psychologically stressed group. Apparent iron absorption was decreased by increased iron supplementation in rats treated with psychological stress more than in control rats. Similarly, iron overload decreased superoxide dismutase activity and apparent iron absorption more significantly in psychologically stressed rats than in controls. Reduced glutathione level varied with diet, increasing in rats on a moderately high-iron diet but decreasing in rats on a extremely high-level iron diet.
These results demonstrated that iron overload augments brain oxidative stress status and aggravates the decrease of apparent iron absorption in a rat model of psychological stress.
研究铁补充对心理应激大鼠脑氧化应激和抗氧化酶活性的影响。
大鼠连续 1 周给予不同铁剂量的饮食，饮食的其他所有成分均根据 AIN-93-G 饮食完全等量匹配。实验期末，处死大鼠并采集脑。为评估铁摄入的影响，测定了血清铁、表观铁吸收率、脑铁浓度、脂质过氧化、还原型谷胱甘肽和超氧化物歧化酶活性。
铁过载显著增加了大鼠脑铁含量和丙二醛水平，尤其是在心理应激组。与对照组相比，心理应激大鼠铁补充增加时，表观铁吸收率降低。同样，铁过载使心理应激大鼠的超氧化物歧化酶活性和表观铁吸收率比对照组显著降低。还原型谷胱甘肽水平随饮食而变化，在高铁饮食组的大鼠中增加，但在高铁水平饮食组的大鼠中降低。
这些结果表明，铁过载加重了心理应激大鼠的脑氧化应激状态，并加重了表观铁吸收率的降低。
