p97 AAA ATPase 的正协同作用对基本功能至关重要。
Positive cooperativity of the p97 AAA ATPase is critical for essential functions.
机构信息
Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
出版信息
J Biol Chem. 2011 May 6;286(18):15815-20. doi: 10.1074/jbc.M110.201400. Epub 2011 Mar 18.
Abstract
p97 is composed of two conserved AAA (ATPases associated with diverse cellular activities) domains, which form a tandem hexameric ring. We characterized the ATP hydrolysis mechanism of CDC-48.1, a p97 homolog of Caenorhabditis elegans. The ATPase activity of the N-terminal AAA domain was very low at physiological temperature, whereas the C-terminal AAA domain showed high ATPase activity in a coordinated fashion with positive cooperativity. The cooperativity and coordination are generated by different mechanisms because a noncooperative mutant still showed the coordination. Interestingly, the growth speed of yeast cells strongly related to the positive cooperativity rather than the ATPase activity itself, suggesting that the positive cooperativity is critical for the essential functions of p97.
摘要
p97 由两个保守的 AAA（与多种细胞活动相关的 ATP 酶）结构域组成，这些结构域形成串联的六聚体环。我们对 CDC-48.1 的 ATP 水解机制进行了表征，CDC-48.1 是秀丽隐杆线虫 p97 的同源物。生理温度下，N 端 AAA 结构域的 ATP 酶活性非常低，而 C 端 AAA 结构域以协调的方式表现出高 ATP 酶活性，且具有正协同性。协同性和协调性是由不同的机制产生的，因为非协同性突变体仍然表现出协调性。有趣的是，酵母细胞的生长速度与正协同性密切相关，而不是与 ATP 酶活性本身相关，这表明正协同性对于 p97 的基本功能至关重要。
相似文献
1
Positive cooperativity of the p97 AAA ATPase is critical for essential functions.
J Biol Chem. 2011 May 6;286(18):15815-20. doi: 10.1074/jbc.M110.201400. Epub 2011 Mar 18.
2
TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching.
Elife. 2015 Apr 28;4:e07367. doi: 10.7554/eLife.07367.
3
Structural insights into the unusually strong ATPase activity of the AAA domain of the Caenorhabditis elegans fidgetin-like 1 (FIGL-1) protein.
J Biol Chem. 2013 Oct 11;288(41):29305-12. doi: 10.1074/jbc.M113.502559. Epub 2013 Aug 26.
4
Characterization of C-terminal adaptors, UFD-2 and UFD-3, of CDC-48 on the polyglutamine aggregation in C. elegans.
Biochem Biophys Res Commun. 2015 Mar 27;459(1):154-60. doi: 10.1016/j.bbrc.2015.02.088. Epub 2015 Feb 24.
5
ATP-bound form of the D1 AAA domain inhibits an essential function of Cdc48p/p97.
Biochem Cell Biol. 2010 Feb;88(1):109-17. doi: 10.1139/o09-116.
6
High-speed atomic force microscopic observation of ATP-dependent rotation of the AAA+ chaperone p97.
Structure. 2013 Nov 5;21(11):1992-2002. doi: 10.1016/j.str.2013.08.017. Epub 2013 Sep 19.
7
CDC-48/p97 is required for proper meiotic chromosome segregation via controlling AIR-2/Aurora B kinase localization in Caenorhabditis elegans.
J Struct Biol. 2012 Aug;179(2):104-11. doi: 10.1016/j.jsb.2012.06.009. Epub 2012 Jun 23.
8
Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription.
EMBO Rep. 2015 Mar;16(3):332-40. doi: 10.15252/embr.201439123. Epub 2015 Feb 4.
9
Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner.
J Cell Sci. 2009 Oct 15;122(Pt 20):3663-72. doi: 10.1242/jcs.052415. Epub 2009 Sep 22.
10
An Afg2/Spaf-related Cdc48-like AAA ATPase regulates the stability and activity of the C. elegans Aurora B kinase AIR-2.
Dev Cell. 2008 Oct;15(4):603-16. doi: 10.1016/j.devcel.2008.08.005.
引用本文的文献
1
Thermodynamic coupling between neighboring binding sites in homo-oligomeric ligand sensing proteins from mass resolved ligand-dependent population distributions.
Protein Sci. 2022 Oct;31(10):e4424. doi: 10.1002/pro.4424.
2
Cryo-EM structures of human p97 double hexamer capture potentiated ATPase-competent state.
Cell Discov. 2022 Feb 22;8(1):19. doi: 10.1038/s41421-022-00379-1.
3
Structural and Functional Analysis of Disease-Linked p97 ATPase Mutant Complexes.
Int J Mol Sci. 2021 Jul 28;22(15):8079. doi: 10.3390/ijms22158079.
4
Significance of Individual Domains of ClpL: A Novel Chaperone from .
Biochemistry. 2020 Sep 15;59(36):3368-3379. doi: 10.1021/acs.biochem.0c00544. Epub 2020 Aug 19.
5
Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7.
Nat Commun. 2019 Jan 31;10(1):513. doi: 10.1038/s41467-019-08373-0.
6
Dsc E3 ligase localization to the Golgi requires the ATPase Cdc48 and cofactor Ufd1 for activation of sterol regulatory element-binding protein in fission yeast.
J Biol Chem. 2017 Sep 29;292(39):16333-16350. doi: 10.1074/jbc.M117.802025. Epub 2017 Aug 18.
7
An information theoretic framework reveals a tunable allosteric network in group II chaperonins.
Nat Struct Mol Biol. 2017 Sep;24(9):726-733. doi: 10.1038/nsmb.3440. Epub 2017 Jul 24.
8
Deviation of the typical AAA substrate-threading pore prevents fatal protein degradation in yeast Cdc48.
Sci Rep. 2017 Jul 14;7(1):5475. doi: 10.1038/s41598-017-05806-y.
9
A Mighty "Protein Extractor" of the Cell: Structure and Function of the p97/CDC48 ATPase.
Front Mol Biosci. 2017 Jun 13;4:39. doi: 10.3389/fmolb.2017.00039. eCollection 2017.
10
Structure and Function of p97 and Pex1/6 Type II AAA+ Complexes.
Front Mol Biosci. 2017 May 29;4:33. doi: 10.3389/fmolb.2017.00033. eCollection 2017.
本文引用的文献
1
ATP-bound form of the D1 AAA domain inhibits an essential function of Cdc48p/p97.
Biochem Cell Biol. 2010 Feb;88(1):109-17. doi: 10.1139/o09-116.
2
AAA+ chaperone ClpX regulates dynamics of prokaryotic cytoskeletal protein FtsZ.
J Biol Chem. 2010 Feb 26;285(9):6648-57. doi: 10.1074/jbc.M109.080739. Epub 2009 Dec 17.
3
An intersubunit signaling network coordinates ATP hydrolysis by m-AAA proteases.
Mol Cell. 2009 Sep 11;35(5):574-85. doi: 10.1016/j.molcel.2009.07.018.
4
p97 Homologs from Caenorhabditis elegans, CDC-48.1 and CDC-48.2, suppress the aggregate formation of huntingtin exon1 containing expanded polyQ repeat.
Genes Cells. 2008 Aug;13(8):827-38. doi: 10.1111/j.1365-2443.2008.01214.x. Epub 2008 Jul 31.
5
Improved structures of full-length p97, an AAA ATPase: implications for mechanisms of nucleotide-dependent conformational change.
Structure. 2008 May;16(5):715-26. doi: 10.1016/j.str.2008.02.010.
6
Analysis of nucleotide binding to P97 reveals the properties of a tandem AAA hexameric ATPase.
J Biol Chem. 2008 May 16;283(20):13745-52. doi: 10.1074/jbc.M709632200. Epub 2008 Mar 10.
7
Protein unfolding by a AAA+ protease is dependent on ATP-hydrolysis rates and substrate energy landscapes.
Nat Struct Mol Biol. 2008 Feb;15(2):139-45. doi: 10.1038/nsmb.1380. Epub 2008 Jan 27.
8
Caenorhabditis elegans p97/CDC-48 is crucial for progression of meiosis I.
Biochem Biophys Res Commun. 2007 Jul 6;358(3):920-4. doi: 10.1016/j.bbrc.2007.05.022. Epub 2007 May 15.
9
Classification of AAA+ proteins.
J Struct Biol. 2006 Oct;156(1):2-11. doi: 10.1016/j.jsb.2006.05.002. Epub 2006 May 26.
10
Central pore residues mediate the p97/VCP activity required for ERAD.
Mol Cell. 2006 May 19;22(4):451-62. doi: 10.1016/j.molcel.2006.03.036.
Zotero 插件