CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Org Lett. 2011 May 6;13(9):2212-5. doi: 10.1021/ol200447h. Epub 2011 Apr 1.
TrdL, encoding a flavin-dependent oxidoreductase in the tirandamycin gene cluster, was inactivated to afford a ΔtrdL mutant, the fermentation of which yielded a new intermediate, tirandamycin E (5), and an additional early intermediate, tirandamycin F (6), if XAD-16 resin was introduced. TrdL was overexpressed in E. coli, and the protein was shown to efficiently catalyze the transformations from 5 to tirandamycin A (1) and from 6 to tirandamycin D (4), demonstrating its function as a 10-hydroxy dehydrogenase.
TrdL，编码替拉霉素基因簇中的黄素依赖氧化还原酶，被失活以获得ΔtrdL 突变体，如果引入 XAD-16 树脂，其发酵会产生新的中间产物替拉霉素 E（5）和另一个早期中间产物替拉霉素 F（6）。TrdL 在大肠杆菌中过表达，并且该蛋白被证明能够有效地催化从 5 到替拉霉素 A（1）和从 6 到替拉霉素 D（4）的转化，证明其作为 10-羟基脱氢酶的功能。
