Eliás B, Herpay M, Albert M, Fekete J, Tuboly S, Rafai P, Molnár E
Department of Research Organization, University of Veterinary Science, Budapest, Hungary.
Vet Microbiol. 1990 Jul;24(1):81-8. doi: 10.1016/0378-1135(90)90053-x.
Colonisation of type D Pasteurella multocida was studied in five groups of seven SPF piglets each. Piglets of Group 1 were kept together with seven 5-week-old piglets obtained from a large herd infected with toxigenic P. multocida for 16 weeks (contact infection). These piglets were made free from toxigenic Bordetella bronchiseptica by local immunisation. Piglets of Group 2 were inoculated with 5 x 10(7) colony-forming units (cfu) of P. multocida washed from the nasal mucosa of piglets free from toxigenic B. bronchiseptica with fetal calf serum. Piglets of Group 3 were inoculated intranasally with 5 x 10(7) cfu of P. multocida washed from yeast-extract proteose-peptone cystine (YPC)-blood agar with fetal calf serum. Piglets of Group 4 were inoculated with 5 x 10(7) cfu of P. multocida grown in a YPC-based broth without blood. Piglets of Group 5 served as controls. The piglets of Group 1 did not contract P. multocida infection from infected contact piglets. After a single inoculation one of four, while after three inoculations two of three piglets of Group 2 became infected by P. multocida. After a single inoculation none of four, while after three inoculations one of three piglets of Group 3 were colonised by P. multocida. Both single and repeated inoculation failed in piglets of Group 4.
对五组每组七只无特定病原体（SPF）仔猪进行了D型多杀性巴氏杆菌定植的研究。第1组仔猪与七只从感染产毒素多杀性巴氏杆菌的大猪群中获得的5周龄仔猪饲养在一起16周（接触感染）。这些仔猪通过局部免疫清除了产毒素支气管败血波氏杆菌。第2组仔猪接种了用胎牛血清从无产毒素支气管败血波氏杆菌的仔猪鼻黏膜冲洗下来的5×10⁷菌落形成单位（cfu）的多杀性巴氏杆菌。第3组仔猪经鼻接种用胎牛血清从酵母提取物蛋白胨胱氨酸（YPC）血琼脂上冲洗下来的5×10⁷cfu的多杀性巴氏杆菌。第4组仔猪接种了在不含血的基于YPC的肉汤中培养的5×10⁷cfu的多杀性巴氏杆菌。第5组仔猪作为对照。第1组仔猪未从感染的接触仔猪感染多杀性巴氏杆菌。单次接种后，第2组四只仔猪中有一只感染，而三次接种后，三只仔猪中有两只感染了多杀性巴氏杆菌。单次接种后，第3组四只仔猪中无一感染，而三次接种后，三只仔猪中有一只被多杀性巴氏杆菌定植。第4组仔猪单次和重复接种均失败。
