Outlaw M C, Armstrong S J, Dimmock N J
Department of Biological Sciences, University of Warwick, Coventry, United Kingdom.
Virology. 1990 Oct;178(2):478-85. doi: 10.1016/0042-6822(90)90345-r.
The interaction of IgG-neutralized type A influenza virus with differentiated epithelial cells of mouse trachea, BHK cells, and chicken erythrocytes was studied using three mouse monoclonal antibodies (IgG2a) each directed against a different antigenic site on the hemagglutinin. At high HIU:HAU ratios virus was neutralized greater than 99%, monodisperse, and attached to tracheal epithelial and BHK cells in normal amounts. The majority (70-80%) of neutralized virus failed to attach to erythrocytes. At low HIU:HAU ratios the virus was aggregated by each of the antibodies, and attachment to tracheal epithelial and BHK cells was inhibited by up to 75%. Combined aggregation and inhibition of attachment could theoretically account for up to 96% loss of infectivity but this corresponded with the observed degree of neutralization with only one of the antibodies. With increasing antibody:virus ratios, aggregation and inhibition of attachment contributed ever diminishingly to the observed neutralization and eventually not at all. Both neutralized and infectious virus attached to neuraminidase-sensitive receptors. After attachment neutralized virus became increasingly resistant to removal by neuraminidase suggesting that it had been internalized by the cell.
利用三种分别针对血凝素上不同抗原位点的小鼠单克隆抗体（IgG2a），研究了IgG中和的A型流感病毒与小鼠气管分化上皮细胞、BHK细胞和鸡红细胞之间的相互作用。在高HIU:HAU比率下，病毒中和率大于99%，呈单分散状态，以正常数量附着于气管上皮细胞和BHK细胞。大多数（70 - 80%）被中和的病毒无法附着于红细胞。在低HIU:HAU比率下，病毒被每种抗体聚集，对气管上皮细胞和BHK细胞的附着抑制高达75%。理论上，聚集和附着抑制共同作用可导致高达96%的感染性丧失，但这仅与其中一种抗体观察到的中和程度相符。随着抗体与病毒比率的增加，聚集和附着抑制对观察到的中和作用的贡献越来越小，最终完全不起作用。中和病毒和感染性病毒均附着于神经氨酸酶敏感受体。附着后，中和病毒对神经氨酸酶去除的抵抗力越来越强，表明它已被细胞内化。
