Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, Florida 33101, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2011;4:297-319. doi: 10.1146/annurev-anchem-061010-113855.
There is an increasing need for versatile yet sensitive labels, posed by the demands for low detection in bioanalysis. Bioluminescent proteins have many desirable characteristics, including the ability to be detected at extremely low concentrations; no background interference from autofluorescent compounds present in samples; and compatibility with many miniaturized platforms, such as lab-on-a-chip and lab-on-a-CD systems. Bioluminescent proteins have found a plethora of analytical applications in intracellular monitoring, genetic regulation and detection, immuno- and binding assays, and whole-cell biosensors, among others. As new bioluminescent organisms are discovered and new bioluminescence proteins are characterized, use of these proteins will continue to dramatically improve our understanding of molecular and cellular events, as well as their applications for detection of environmental and biomedical samples.
生物分析中对低检测限的需求不断增加，因此需要多功能且灵敏的标签。生物发光蛋白具有许多理想的特性，包括能够检测极低浓度的物质；样品中存在的自发荧光化合物不会产生背景干扰；并且与许多小型化平台（如芯片实验室和 CD 实验室系统）兼容。生物发光蛋白在细胞内监测、遗传调控和检测、免疫和结合分析以及全细胞生物传感器等方面有很多分析应用。随着新的生物发光生物的发现和新的生物发光蛋白的特性化，这些蛋白的使用将继续极大地提高我们对分子和细胞事件的理解，以及它们在环境和生物医学样品检测中的应用。
