LTB Lasertechnik Berlin, Berlin, Germany.
Pigment Cell Melanoma Res. 2011 Jun;24(3):438-45. doi: 10.1111/j.1755-148X.2011.00853.x. Epub 2011 May 2.
Malignant transformation of melanocytes is associated with changes in melanogenesis. Therefore, fluorescence of melanin may be an informative indicator of this process. But the conventionally excited autofluorescence of melanin in skin tissue is ultra-weak and its main part in the visible spectral region is hidden by the much stronger fluorescence from other endogenous fluorophores. Here, using a new mode of stepwise two-photon excitation, melanin-dominated fluorescence spectra of pigmented skin lesions are reported. From these, pure melanin fluorescence spectra of normal pigmented skin, melanocytic nevi and malignant pigmented melanoma were analyzed. They show distinctly different spectral shapes: melanoma gave a characteristic fingerprint with a fluorescence band peaking at 640 nm, independent of the melanoma subtype. The melanin fluorescence spectra peaked at 590 nm for all types of common melanocytic nevi. These differences in the fluorescence spectra are probably based on different contents of eumelanin and pheomelanin. In a series of 167 cases with melanocytic nevi and melanomas, the sensitivity of this new method to diagnose melanoma was 93.5%, the specificity 80.0% and the diagnostic accuracy 82.6%. The two-photon excitation fluorescence method is a new diagnostic tool which may in future supplement conventional dermatohistopathology.
黑色素细胞的恶性转化与黑色素生成的变化有关。因此，黑色素的荧光可能是该过程的一个信息指标。但是，皮肤组织中黑色素的传统激发自发荧光非常微弱，其在可见光谱区域的主要部分被其他内源性荧光团的强荧光所掩盖。在这里，我们使用一种新的逐步双光子激发模式，报道了色素性皮肤病变中以黑色素为主的荧光光谱。从中，我们分析了正常色素皮肤、黑素细胞痣和恶性色素性黑色素瘤的纯黑色素荧光光谱。它们显示出明显不同的光谱形状：黑色素瘤表现出特征指纹，荧光带在 640nm 处峰值，与黑色素瘤亚型无关。所有常见的黑素细胞痣的黑色素荧光光谱峰值均在 590nm。这些荧光光谱的差异可能基于真黑素和褐黑素的含量不同。在 167 例黑素细胞痣和黑色素瘤的病例系列中，这种新方法诊断黑色素瘤的灵敏度为 93.5%，特异性为 80.0%，诊断准确性为 82.6%。双光子激发荧光法是一种新的诊断工具，它可能在未来补充传统的皮肤组织病理学。
