University of Szeged, Faculty of Medicine, Department of Pathophysiology, Semmelweis 1, Szeged, Hungary.
Behav Brain Res. 2011 Sep 23;222(2):295-8. doi: 10.1016/j.bbr.2011.03.047. Epub 2011 Mar 30.
In the present experiments, the anxiolytic action of Ucn 3 fragments were studied in an elevated plus maze in mice following icv administration in an attempt to identify the biologically active center of the molecule. Ucn 3, Ucn 3 (18-38), Ucn 3 (19-27), Ucn 3 (28-38), Ucn 3 (34-38), and Ucn 3 (36-38) but not Ucn 3 (34-36), demonstrated anxiolytic action by increasing the time the mice spent in open arms, and Ucn 3 and Ucn 3 (34-38) did so by increasing the number of entries into open arms. The shortest molecule which elicited anxiolytic effects was the tripeptide Ucn 3 (36-38), H-Ala-Gln-Ile-NH(2). The results indicate that the total sequence of Ucn 3 is not necessary for the anxiolytic action of Ucn 3. Establishment the smallest active sequence of Ucn 3 will allow the synthesis of analogs or mimetics for anxiolytic action.
在目前的实验中，通过脑室给药，研究了 Ucn 3 片段在高架十字迷宫中的抗焦虑作用，试图确定该分子的生物活性中心。Ucn 3、Ucn 3（18-38）、Ucn 3（19-27）、Ucn 3（28-38）、Ucn 3（34-38）和 Ucn 3（36-38）但不是 Ucn 3（34-36），通过增加小鼠在开放臂上的时间表现出抗焦虑作用，Ucn 3 和 Ucn 3（34-38）通过增加进入开放臂的次数来实现。引起抗焦虑作用的最短分子是三肽 Ucn 3（36-38），H-Ala-Gln-Ile-NH（2）。结果表明，Ucn 3 的全长序列对于 Ucn 3 的抗焦虑作用不是必需的。确定 Ucn 3 的最小活性序列将允许合成具有抗焦虑作用的类似物或模拟物。
