Department of Resources and Environmental Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan.
Biomaterials. 2011 Jul;32(21):4896-902. doi: 10.1016/j.biomaterials.2011.03.021. Epub 2011 Apr 1.
Gene transfer techniques are useful tools for controlling cell behavior, such as proliferation and differentiation. We have recently developed an efficient area-specific gene transfer system using a DNA-fibronectin-apatite composite layer (DF-Ap layer). In this system, partial dissolution of the composite layer is likely to be a crucial step for gene transfer. In the present study, layer solubility was adjusted by incorporating various contents of carbonate or fluoride ions into the DF-Ap layer via ionic substitution for the apatite crystals. Carbonate ion incorporation increased the solubility of the DF-Ap layer, thereby increasing the efficiency of gene transfer on the layer. In contrast, the incorporation of fluoride ions decreased the solubility of the DF-Ap layer, thereby decreasing the efficiency and delaying the timing of gene transfer on the layer dose-dependently. The present gene transfer system with controllable efficiency and timing would be useful in tissue engineering applications because cell differentiation can be induced effectively by regulating appropriate gene expression with suitable timing.
基因转移技术是控制细胞行为（如增殖和分化）的有用工具。我们最近开发了一种使用 DNA-纤维连接蛋白-磷灰石复合层（DF-Ap 层）的高效区域特异性基因转移系统。在该系统中，复合层的部分溶解可能是基因转移的关键步骤。在本研究中，通过离子取代磷灰石晶体将各种含量的碳酸盐或氟化物离子掺入 DF-Ap 层中，从而调整层的溶解度。碳酸根离子的掺入增加了 DF-Ap 层的溶解度，从而提高了基因转移到该层上的效率。相比之下，氟化物离子的掺入降低了 DF-Ap 层的溶解度，从而降低了基因转移到该层上的效率，并使基因转移的时间滞后，呈剂量依赖性。这种具有可控效率和时间的新型基因转移系统在组织工程应用中可能很有用，因为可以通过适当的时间调节适当的基因表达来有效地诱导细胞分化。
