槲皮素固定化二氧化钛促进新骨形成：载药方式对体内、体外骨髓基质细胞分化的影响。
Kaempferol-immobilized titanium dioxide promotes formation of new bone: effects of loading methods on bone marrow stromal cell differentiation in vivo and in vitro.
机构信息
Department of Oral and Maxillofacial Surgery, Nagoya University Hospital, Nagoya, Japan.
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
出版信息
Int J Nanomedicine. 2018 Mar 19;13:1665-1676. doi: 10.2147/IJN.S150786. eCollection 2018.
BACKGROUND
Surface modification of titanium dioxide (TiO) implants promotes bone formation and shortens the osseointegration period. Kaempferol is a flavonoid that has the capacity to promote osteogenic differentiation in bone marrow stromal cells. The aim of this study was to promote bone formation around kaempferol immobilized on TiO implants.
METHODS
There were four experimental groups. Alkali-treated TiO samples (implants and discs) were used as a control and immersed in Dulbecco's phosphate-buffered saline (DPBS) (Al-Ti). For the coprecipitation sample (Al-cK), the control samples were immersed in DPBS containing 50 µg kaempferol/100% ethanol. For the adsorption sample (Al-aK), 50 µg kaempferol/100% ethanol was dropped onto control samples. The surface topography of the TiO implants was observed by scanning electron microscopy with energy-dispersive X-ray spectroscopy, and a release assay was performed. For in vitro experiments, rat bone marrow stromal cells (rBMSCs) were cultured on each of the TiO samples to analyze cell proliferation, alkaline phosphatase activity, calcium deposition, and osteogenic differentiation. For in vivo experiments, TiO implants placed on rat femur bones were analyzed for bone-implant contact by histological methods.
RESULTS
Kaempferol was detected on the surface of Al-cK and Al-aK. The results of the in vitro study showed that rBMSCs cultured on Al-cK and Al-aK promoted alkaline phosphatase activity, calcium deposition, and osteogenic differentiation. The in vivo histological analysis revealed that Al-cK and Al-aK stimulated new bone formation around implants.
CONCLUSION
TiO implant-immobilized kaempferol may be an effective tool for bone regeneration around dental implants.
背景
二氧化钛（TiO）植入物的表面改性可促进骨形成并缩短骨整合期。山奈酚是一种类黄酮，具有促进骨髓基质细胞成骨分化的能力。本研究旨在促进山奈酚固定在 TiO 植入物周围的骨形成。
方法
有四个实验组。碱处理的 TiO 样品（植入物和圆盘）用作对照并浸入 Dulbecco 的磷酸盐缓冲盐水（DPBS）（Al-Ti）中。对于共沉淀样品（Al-cK），对照样品浸入含有 50 µg 山奈酚/100%乙醇的 DPBS 中。对于吸附样品（Al-aK），将 50 µg 山奈酚/100%乙醇滴加到对照样品上。通过扫描电子显微镜和能量色散 X 射线光谱观察 TiO 植入物的表面形貌，并进行释放试验。在体外实验中，将大鼠骨髓基质细胞（rBMSCs）培养在每种 TiO 样品上，以分析细胞增殖、碱性磷酸酶活性、钙沉积和成骨分化。在体内实验中，通过组织学方法分析放置在大鼠股骨上的 TiO 植入物的骨-植入物接触情况。
结果
在 Al-cK 和 Al-aK 表面检测到山奈酚。体外研究结果表明，rBMSCs 在 Al-cK 和 Al-aK 上培养可促进碱性磷酸酶活性、钙沉积和成骨分化。体内组织学分析表明，Al-cK 和 Al-aK 可刺激植入物周围的新骨形成。
结论
TiO 植入物固定的山奈酚可能是促进牙种植体周围骨再生的有效工具。
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