Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110 Pretoria, South Africa.
Vet Microbiol. 2011 Jul 5;151(1-2):139-47. doi: 10.1016/j.vetmic.2011.02.037. Epub 2011 Feb 24.
From 2005 to 2007, Mycobacterium tuberculosis complex (MTC) strains were isolated from cattle, goats and pigs samples collected at the Bodija abattoir and from human samples from tuberculosis patients and livestock traders at the Akinyele cattle market in Ibadan, Southwestern Nigeria. Seventy four isolates obtained from humans (24) and livestock (50) were identified as MTC strains. Thirty two isolates were spoligotyped. Nineteen of these 32 isolates were identified as M. tuberculosis whilst 13 were identified as Mycobacterium bovis. M. bovis was isolated from two humans, whereas M. tuberculosis was isolated from a bovine, a pig and a goat. All the M. bovis isolates identified in this study belonged to the Africa 1 clonal complex. Multiple locus VNTR [variable number of tandem repeats] analysis (MLVA) was carried out on the 74 isolates. Three major clusters were defined. Group A consisted of 24 M. tuberculosis isolates (MLVA genotypes 1-18). One strain was isolated from a bovine and one from a pig. Group B consisted of 49 M. bovis strains (MLVA genotypes 19-48), mainly of cattle origin but also included four goat, nine pig and two human isolates. Group C consisted of a single M. tuberculosis isolate (MLVA genotype 49) obtained from a goat. Spoligotyping and MLVA confirmed it as clustering with the East Africa Indian clade found in humans in Sudan and the Republic of Djibouti. The isolation of three M. tuberculosis strains from livestock raises the question of their epidemiological importance as a source of infection for humans.
从 2005 年到 2007 年，在尼日利亚西南部伊巴丹的博迪贾屠宰场从牛、山羊和猪的样本中，以及从结核病患者和牲畜交易商的人体样本中分离出结核分枝杆菌复合群（MTC）菌株。从人类（24 例）和牲畜（50 例）中获得的 74 株分离物被鉴定为 MTC 菌株。对 32 株分离物进行了 spoligotyping 分析。这 32 株分离物中有 19 株被鉴定为结核分枝杆菌，13 株被鉴定为牛分枝杆菌。本研究中分离出的牛分枝杆菌有两株来自人类，而结核分枝杆菌则来自牛、猪和山羊。本研究中鉴定的所有牛分枝杆菌均属于非洲 1 克隆复合体。对 74 株分离物进行了多位点可变数目串联重复分析（MLVA）。定义了三个主要聚类。A 组由 24 株结核分枝杆菌组成（MLVA 基因型 1-18）。其中一株从牛中分离，一株从猪中分离。B 组由 49 株牛分枝杆菌组成（MLVA 基因型 19-48），主要来源于牛，但也包括四株山羊、九株猪和两株人类分离物。C 组由一株来自山羊的结核分枝杆菌分离物（MLVA 基因型 49）组成。 spoligotyping 和 MLVA 证实其与在苏丹和吉布提共和国发现的人类中的东非印度分支聚类。从牲畜中分离出三株结核分枝杆菌，这引发了一个问题，即它们作为人类感染源的流行病学重要性。
