Food Allergy Center and Center for Immunology and Inflammatory Diseases at Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
J Allergy Clin Immunol. 2011 Apr;127(4):843-9; quiz 850-1. doi: 10.1016/j.jaci.2011.02.011.
The development of protein microarray-based immunoassays and the availability of recombinant allergens have, to a significant extent, emerged together over the past decade. Their long-anticipated wider application to allergy diagnosis has recently begun to accelerate. This review discusses some of the strengths and weaknesses of molecularly defined allergy testing and the microarray platform. Several recent applications of microarray assays to allergy testing are also summarized. Promising findings, particularly in the context of food and latex allergy, point to the potential for greater resolution between clinical reactivity and asymptomatic sensitization with this platform.
基于蛋白质微阵列的免疫分析的发展以及重组过敏原的出现，在过去十年中都取得了重大进展。最近，人们开始加速将这些技术更广泛地应用于过敏诊断。本文讨论了分子定义的过敏测试和微阵列平台的一些优缺点。还总结了微阵列分析在过敏测试中的一些最新应用。有希望的发现，特别是在食物和乳胶过敏方面，表明该平台在临床反应与无症状致敏之间具有更高的分辨率的潜力。
