Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN, USA.
Cell Immunol. 2011;269(1):46-53. doi: 10.1016/j.cellimm.2011.03.009. Epub 2011 Mar 15.
We tested the contribution of four staphylococcal components - PSM-α, PSM-β, δ-toxin, and PVL - in triggering macrophage secretion of tumor necrosis factor (TNF) and interleukins 6 (IL-6) and 12 (IL-12) by two prominent, circulating strains of community-associated, methicillin-resistant Staphylococcus aureus (CA-MRSA): LAC, USA300; MW2, USA400. RAW 264.7 murine macrophages were stimulated with live, antibiotic-exposed bacteria, and cytokine secretion was quantitated in supernatants. Deletion of PSM-α expression in LAC led to >50% reduction in macrophage TNF and IL-6 secretion and a 20% reduction in IL-12 secretion, while PSM-α deletion in MW2 did not significantly reduce macrophage TNF secretion but resulted in a 15-20% reduction in IL-6 and IL-12 secretion. Deletion of δ-toxin in either strain led to more than 50% reduction in macrophage IL-6 secretion and smaller reductions in macrophage TNF and IL-12 secretion (8-25%). Our data implicate both PSM-α and δ-toxin in stimulating macrophage cytokine responses to CA-MRSA bacteria.
我们测试了四个葡萄球菌成分——PSM-α、PSM-β、δ-毒素和 PVL——在触发巨噬细胞分泌肿瘤坏死因子 (TNF)、白细胞介素 6 (IL-6) 和白细胞介素 12 (IL-12) 方面的作用，这是两种常见的、循环的耐甲氧西林金黄色葡萄球菌 (CA-MRSA) 菌株：LAC，USA300；MW2，USA400。RAW 264.7 鼠巨噬细胞用活的、暴露于抗生素的细菌刺激，并在培养上清液中定量细胞因子的分泌。LAC 中 PSM-α 表达的缺失导致巨噬细胞 TNF 和 IL-6 分泌减少>50%，而 IL-12 分泌减少 20%，而 MW2 中 PSM-α 的缺失并未显著减少巨噬细胞 TNF 的分泌，但导致 IL-6 和 IL-12 分泌减少 15-20%。两种菌株中 δ-毒素的缺失导致巨噬细胞 IL-6 分泌减少>50%，而 TNF 和 IL-12 分泌减少 8-25%。我们的数据表明 PSM-α 和 δ-毒素都参与了刺激 CA-MRSA 细菌引起的巨噬细胞细胞因子反应。
