Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Microbiol Res. 2010 Feb 28;165(2):152-62. doi: 10.1016/j.micres.2009.03.005. Epub 2009 Jul 17.
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+ methicillin-susceptible S. aureus and PVL+CA-MRSA from different countries, and investigated the allelic variations among their lukS-PV and lukF-PV gene sequences. The prevalence of PVL+MRSA was 19.04%. They belonged to different genetic clones with multi-locus sequence types (MLST) 30, 80, and the novel type 1010. ST80 strains showed unique antibiotic resistance profile that was distinguishable from the European ST80 clone: they had no resistance to tetracycline and fusidic acid. Two nonsynonymous substitutions in the lukS-PV gene region and two haplotype variants among these isolates were detected. We revealed that PVL is a good marker for CA-MRSA infections and that PVL+ isolates belonged to different genotypes.
在世界范围内已经对表达杀白细胞素（PVL）的社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）进行了研究，但在埃及尚未进行此类研究。在本研究中，我们分析了埃及的 PVL+CA-MRSA 的分子特征，比较了它们的遗传模式与 PVL+甲氧西林敏感金黄色葡萄球菌和来自不同国家的 PVL+CA-MRSA 的遗传模式，并研究了它们 lukS-PV 和 lukF-PV 基因序列之间的等位基因变异。PVL+MRSA 的流行率为 19.04%。它们属于不同的遗传克隆，具有多基因序列类型（MLST）30、80 和新型 1010。ST80 株表现出独特的抗生素耐药谱，与欧洲 ST80 克隆不同：它们对四环素和夫西地酸没有耐药性。在 lukS-PV 基因区域检测到两个非同义突变和这些分离株中的两个单倍型变异。我们揭示了 PVL 是 CA-MRSA 感染的良好标志物，并且 PVL+分离株属于不同的基因型。
