Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Paraná, Brazil.
Hospital Universitário, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
J Med Microbiol. 2011 Jul;60(Pt 7):1026-1031. doi: 10.1099/jmm.0.028597-0. Epub 2011 Apr 1.
A total of 41 Salmonella Enteritidis strains, including phago-types (PTs) PT4 and PT9, were characterized by antimicrobial resistance profiles and PFGE. Of these strains, 34 were isolated from patients and foods, and 7 were of poultry origin. All strains were susceptible to ampicillin, chloramphenicol, cefotaxime, ciprofloxacin and trimethoprim/sulfamethoxazole, and 41.5 % (n = 17) were resistant to nalidixic acid. PFGE analysis using XbaI and SpeI restriction enzymes resulted in X1S1 as the prevalent pattern, which was present in 48.8 % (n = 20) of epidemic strains and in one strain isolated from discarded hatching eggs. Distinct patterns were found for the other strains isolated from poultry (X3S1, X8S8, X11S12, X11S13, X16S1 and X13S15). The S. Enteritidis PT9 strains associated with outbreaks of salmonellosis were highly similar (≥0.90), suggesting clonality. The PFGE genotypes were related to the PTs, and it was possible to differentiate strains isolated from patients with salmonellosis from other strains of non-epidemic origin. The PFGE results suggested that the S. Enteritidis strains of poultry origin were a possible source of human salmonellosis during the study period.
共 41 株肠炎沙门氏菌分离株，包括噬菌体型（PT）PT4 和 PT9，通过抗菌药物耐药谱和 PFGE 特征进行了鉴定。这些分离株中，34 株来自患者和食品，7 株来自禽类。所有菌株均对氨苄西林、氯霉素、头孢噻肟、环丙沙星和复方磺胺甲噁唑敏感，41.5%（n=17）对萘啶酸耐药。XbaI 和 SpeI 内切酶的 PFGE 分析显示，X1S1 是主要流行模式，在 48.8%（n=20）的流行菌株和 1 株来自废弃孵化蛋的菌株中存在。从禽类分离的其他菌株存在不同的模式（X3S1、X8S8、X11S12、X11S13、X16S1 和 X13S15）。与肠炎沙门氏菌感染暴发相关的 PT9 株高度相似（≥0.90），提示其具有克隆性。PFGE 基因型与 PT 相关，并且可以将来自肠炎沙门氏菌感染患者的菌株与非流行来源的其他菌株区分开来。PFGE 结果表明，在研究期间，来自禽类的肠炎沙门氏菌菌株可能是人类沙门氏菌病的一个潜在来源。
