Song Qifa, Shen Xuanyi, Yang Yuanbin, Zhang Danyang, Gao Hong
Dept. of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention, Ningbo, Zhejiang, People's Republic of China.
J Food Sci. 2016 Jul;81(7):M1778-81. doi: 10.1111/1750-3841.13339. Epub 2016 May 26.
Salmonella enterica serotype Enteritidis (S. Enteritidis) is an important causative agent of nontyphoidal salmonellosis in human populations. In this study, we collected 72 S. Enteritidis strains from 2004 to 2014 in Ningbo, mid-east China. Of the 72 strains, we identified a dominant clone of 58 strains recovered from patient's feces (n = 48), blood (n = 1), pleural effusion (n = 1), chickens (n = 3), and dessert cakes (n = 5) by pulsed-field gel electrophoresis (PFGE) and variable-number of tandem repeat analysis (MLVA). The profile arrangements of MLVA were SE1-SE2-SE3-SE5-SE6-SE8-SE9: 4-4-3-11-10-1-3. These dominant strains were susceptible to ampicillin, chloramphenicol, tetracycline, ciprofloxacin, gentamicin, cefotaxime and trimethoprim-sulfamethoxazole, and resistant to nalidixic acid. Additionally, all isolates harboured virulence genes invA, sipA, sopE, and spvB when tested by PCR. Our results reveal that genetically similar S. Enteritidis strains which accounted for several outbreaks as well as blood infection and pleural cavity infection are prevalent in China for a long-term period. This situation calls for further attention in the prevention and control of foodborne disease caused by Salmonella species.
肠炎沙门氏菌肠炎血清型（肠炎沙门氏菌）是人类非伤寒型沙门氏菌病的重要病原体。在本研究中，我们于2004年至2014年在中国东部的宁波收集了72株肠炎沙门氏菌菌株。在这72株菌株中，我们通过脉冲场凝胶电泳（PFGE）和可变数目串联重复序列分析（MLVA）从患者粪便（n = 48）、血液（n = 1）、胸腔积液（n = 1）、鸡（n = 3）和甜点蛋糕（n = 5）中鉴定出58株菌株的一个优势克隆。MLVA的图谱排列为SE1-SE2-SE3-SE5-SE6-SE8-SE9：4-4-3-11-10-1-3。这些优势菌株对氨苄青霉素、氯霉素、四环素、环丙沙星、庆大霉素、头孢噻肟和甲氧苄啶-磺胺甲恶唑敏感，对萘啶酸耐药。此外，通过PCR检测时，所有分离株都携带毒力基因invA、sipA、sopE和spvB。我们的结果表明，在中国长期存在遗传相似的肠炎沙门氏菌菌株，这些菌株导致了几起疫情以及血液感染和胸腔感染。这种情况在预防和控制由沙门氏菌引起的食源性疾病方面需要进一步关注。
