Department of Productive Plants and Interaction with the Environment (DIPIA), National Institute of Occupational Safety and Prevention (exISPESL-INAIL), Via Urbana, 167 - 00184 Rome, Italy.
Department of Environmental Biology, Sapienza University of Rome, P.le A. Moro, 5 - 00185 Rome, Italy.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):451-458. doi: 10.1099/ijs.0.031682-0. Epub 2011 Apr 1.
The morphology of three phenetically identical Penicillium isolates, collected from the bioaerosol in a restoration laboratory in Italy, displayed macro- and microscopic characteristics that were similar though not completely ascribable to Penicillium raistrickii. For this reason, a phylogenetic approach based on DNA sequencing analysis was performed to establish both the taxonomic status and the evolutionary relationships of these three peculiar isolates in relation to previously described species of the genus Penicillium. We used four nuclear loci (both rRNA and protein coding genes) that have previously proved useful for the molecular investigation of taxa belonging to the genus Penicillium at various evolutionary levels. The internal transcribed spacer region (ITS1-5.8S-ITS2), domains D1 and D2 of the 28S rDNA, a region of the tubulin beta chain gene (benA) and part of the calmodulin gene (cmd) were amplified by PCR and sequenced. Analysis of the rRNA genes and of the benA and cmd sequence data indicates the presence of three isogenic isolates belonging to a genetically distinct species of the genus Penicillium, here described and named Penicillium simile sp. nov. (ATCC MYA-4591(T) = CBS 129191(T)). This novel species is phylogenetically different from P. raistrickii and other related species of the genus Penicillium (e.g. Penicillium scabrosum), from which it can be distinguished on the basis of morphological trait analysis.
从意大利一个修复实验室的生物气溶胶中收集到的三个表型相同的青霉分离株的形态，显示出宏观和微观特征，这些特征虽然与 Penicillium raistrickii 相似，但不完全相同。因此，我们采用基于 DNA 测序分析的系统发育方法，确定了这三个特殊分离株的分类地位和进化关系，以及它们与先前描述的 Penicillium 属物种的关系。我们使用了四个核基因座（rRNA 和蛋白质编码基因），这些基因座以前在不同进化水平上对属于 Penicillium 属的分类群的分子研究证明是有用的。内部转录间隔区（ITS1-5.8S-ITS2）、28S rDNA 的 D1 和 D2 结构域、微管蛋白β链基因（benA）的一个区域和钙调蛋白基因（cmd）的一部分通过 PCR 扩增和测序。rRNA 基因和 benA 和 cmd 序列数据的分析表明，存在三个属于青霉属中遗传上不同的种的同基因分离株，我们在这里描述并命名为 Penicillium simile sp. nov.（ATCC MYA-4591(T) = CBS 129191(T)）。这个新种在系统发育上与 P. raistrickii 和其他相关的 Penicillium 属物种（如 Penicillium scabrosum）不同，从形态特征分析可以将其与这些物种区分开来。
