Ouhibi Salma, Santos Carla, Ghali Ridha, Soares Célia, Hedhili Abderrazek, Paterson Russell, Lima Nelson
1Mahmoud Yaacoub Center of Urgent Medical Assistance of Tunis, Laboratory of Toxicology and Environment (LR12SP07) and El Manar University, Tunis, Tunisia.
2CEB-Centre of Biological Engineering, Micoteca da Universidade do Minho (MUM), University of Minho, Braga, Portugal.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3217-3225. doi: 10.1099/ijsem.0.002962. Epub 2018 Aug 20.
Two similar Penicillium isolates could not be identified as previously described species in a survey of orchard apples from Tunisia for patulin-producing fungi. These isolates are described as novel species using multilocus DNA sequence analysis of partial β-tubulin, calmodulin and nuclear ribosomal internal transcribed spacer regions; and morphological, physiological and biochemical characteristics. The isolates were considered negative for patulin production since the IDH gene fragment was not detected and the compound detected at the same retention time of patulin (14.9 min) showed a different UV spectrum using U-HPLC/UV-DAD. In terms of phylogeny, the two isolates clustered with Penicillium section Ramosa and are closely related to Penicillium chroogomphum, Penicillium lenticrescens and Penicillium soppii. Furthermore, their macro- and micromorphological traits differed from these species. Hence, the isolates represent a novel species in Penicillium section Ramosa and the name Penicillium tunisiense sp. nov. is proposed, with the type strain MUM 17.62 (=ITEM 17445).
在对突尼斯果园苹果中产生展青霉素的真菌进行的一项调查中，两种相似的青霉分离株无法鉴定为先前描述的物种。利用部分β-微管蛋白、钙调蛋白和核糖体核糖体内转录间隔区的多位点DNA序列分析，以及形态学、生理学和生化特征，将这些分离株描述为新物种。由于未检测到IDH基因片段，且在展青霉素相同保留时间（14.9分钟）检测到的化合物使用U-HPLC/UV-DAD显示出不同的紫外光谱，因此这些分离株被认为不产生展青霉素。在系统发育方面，这两种分离株与青霉属的Ramosa组聚类，并且与嗜蓝青霉、渐生青霉和索氏青霉密切相关。此外，它们的宏观和微观形态特征与这些物种不同。因此，这些分离株代表了青霉属Ramosa组中的一个新物种，并提出了突尼斯青霉新种（Penicillium tunisiense sp. nov.），其模式菌株为MUM 17.62（=ITEM 17445）。
