Raman Aparna, Gawalt Ellen S
Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, PA 15282.
Mater Sci Eng C Mater Biol Appl. 2010 Oct 12;30(8):1157-1161. doi: 10.1016/j.msec.2010.06.013.
Inhibiting the non-specific adhesion of cells and proteins to biomaterials such as stents, catheters and guide wires is an important interfacial issue that needs to be addressed in order to reduce surface-related implant complications. Medical grade stainless steel 316L was used as a model system to address this issue. To alter the interfacial property of the implant, self assembled monolayers of long chain phosphonic acids with -CH(3), -COOH, -OH tail groups were formed on the native oxide surface of medical grade stainless steel 316L. The effect of varying the tail groups on 3T3 fibroblast adhesion was investigated. The methyl terminated phosphonic acid significantly prevented cell adhesion however presentation of hydrophilic tail groups at the interface did not significantly reduce cell adhesion when compared to the control stainless steel 316L.
抑制细胞和蛋白质与诸如支架、导管和导丝等生物材料的非特异性粘附是一个重要的界面问题，为减少与表面相关的植入物并发症，这一问题需要得到解决。医用级316L不锈钢被用作一个模型系统来解决这个问题。为改变植入物的界面性质，在医用级316L不锈钢的原生氧化物表面形成了具有-CH(3)、-COOH、-OH尾基的长链膦酸自组装单分子层。研究了尾基变化对3T3成纤维细胞粘附的影响。甲基封端的膦酸显著阻止了细胞粘附，然而与对照不锈钢316L相比，界面处亲水性尾基的存在并没有显著降低细胞粘附。
