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基于链霉亲和素-辣根过氧化物酶功能化碳纳米管的信号放大用于检测纳摩尔 DNA 的安培检测

Signal amplification of streptavidin-horseradish peroxidase functionalized carbon nanotubes for amperometric detection of attomolar DNA.

机构信息

Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, PR China.

出版信息

Chem Commun (Camb). 2011 May 14;47(18):5220-2. doi: 10.1039/c1cc10840a. Epub 2011 Apr 2.

DOI:10.1039/c1cc10840a
PMID:21461429
Abstract

A novel biosensing strategy for selective electrochemical detection of DNA down to the attomolar level with a linear range of 5 orders of magnitude was developed by the specific recognitions of target DNA and streptavidin to biotin labelled molecular beacon and signal amplification of streptavidin-horseradish peroxidase functionalized carbon nanotubes.

摘要

开发了一种新的生物传感策略，通过目标 DNA 与链霉亲和素对生物素标记的分子信标特异性识别以及链霉亲和素-辣根过氧化物酶功能化碳纳米管的信号放大，实现了对 DNA 的选择性电化学检测，检测下限达到飞摩尔级，线性范围为 5 个数量级。

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