College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Biol Trace Elem Res. 2011 Dec;144(1-3):538-49. doi: 10.1007/s12011-011-9024-y. Epub 2011 Apr 2.
The aim of the present study was to find out the effects of boron on ostrich chicks fed with 0 mg/l, 100 mg/l, 200 mg/l, and 400 mg/l of additional boron in water. We measured bone mineral density (BMD), perimeter, length, weight, ash content of ostrich tibias, thickness of cortical bone, and diameter of the marrow cavity. We also analyzed the apoptosis status of paraffin sections using a TUNEL kit and examined serum levels of leptin and estradiol (E(2)). The results were dramatic. Compared with the control group, group C had a very high BMD. The serum levels of leptin in groups C and D were significantly higher than control values, and the levels of E(2) fluctuated. The perimeter, length, weight, and ash content of ostrich tibias all increased significantly with increasing dosage of boron. The cross-section analysis revealed that the bone marrow cavity shifted closer to one side in group D, which was observed on a macro-scale. This shift may be related to the toxicity of excessive boron, as indicated by the apoptosis status. According to the present data, additional boron was helpful for ostrich chick bone development, and 200 mg/l supplement boron in the drinking water appeared to be the most beneficial.
本研究旨在探讨在水中添加 0mg/L、100mg/L、200mg/L 和 400mg/L 硼对鸵鸟雏鸡的影响。我们测量了鸵鸟胫骨的骨矿物质密度（BMD）、周长、长度、重量、灰分含量、皮质骨厚度和骨髓腔直径。我们还使用 TUNEL 试剂盒分析了石蜡切片的凋亡状态，并检测了血清瘦素和雌二醇（E(2)）水平。结果非常显著。与对照组相比，C 组的 BMD 非常高。C 组和 D 组的血清瘦素水平明显高于对照组，E(2)水平波动。随着硼剂量的增加，鸵鸟胫骨的周长、长度、重量和灰分含量均显著增加。横截面分析显示，D 组的骨髓腔更靠近一侧，这在宏观尺度上是可以观察到的。这种移位可能与过量硼的毒性有关，这一点可以从凋亡状态中得到证实。根据目前的数据，额外的硼对鸵鸟雏鸡的骨骼发育有帮助，在饮用水中添加 200mg/L 的硼似乎是最有益的。
