Ourliac-Garnier Isabelle, Londoño-Vallejo Arturo
Telomeres & Cancer Laboratory, Institut Curie, Paris, France.
Methods Mol Biol. 2011;735:21-31. doi: 10.1007/978-1-61779-092-8_3.
Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high-affinity probes for telomeric repeated sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.
长度是端粒的一个功能参数，端粒是保护染色体末端的核蛋白结构。用于端粒重复序列的高特异性、高亲和力探针的出现，使得直接在染色体上或间期细胞核中测量端粒长度的定量方法得以发展。在此，我们描述一种在中期染色体上进行端粒定量荧光原位杂交的通用方法，并讨论其在研究中最常见的应用。
