Ourliac-Garnier Isabelle, Londoño-Vallejo Arturo
Telomeres & Cancer Laboratory, Institut Curie, Paris, France.
Methods Mol Biol. 2011;735:33-46. doi: 10.1007/978-1-61779-092-8_4.
The implementation of quantitative approaches in telomere chromosome-oriented FISH (telomeric CO-FISH) allows the assessment of the relative efficiency of lagging versus leading strand telomere replication and thus provides information on the implicated mechanisms. Here, we describe a simple method for telomere strand-specific analyses and discuss its potential applications.
在端粒染色体定向荧光原位杂交（端粒CO-FISH）中采用定量方法，能够评估滞后链与前导链端粒复制的相对效率，从而提供有关相关机制的信息。在此，我们描述一种用于端粒链特异性分析的简单方法，并讨论其潜在应用。
