通过荧光原位杂交进行端粒链特异性长度分析（定量共聚焦荧光原位杂交，Q-CO-FISH）。
Telomere strand-specific length analysis by fluorescent in situ hybridization (Q-CO-FISH).
作者信息
Ourliac-Garnier Isabelle, Londoño-Vallejo Arturo
机构信息
Telomeres & Cancer Laboratory, Institut Curie, Paris, France.
出版信息
Methods Mol Biol. 2011;735:33-46. doi: 10.1007/978-1-61779-092-8_4.
PMID:21461809
Abstract
The implementation of quantitative approaches in telomere chromosome-oriented FISH (telomeric CO-FISH) allows the assessment of the relative efficiency of lagging versus leading strand telomere replication and thus provides information on the implicated mechanisms. Here, we describe a simple method for telomere strand-specific analyses and discuss its potential applications.
摘要
在端粒染色体定向荧光原位杂交（端粒CO-FISH）中采用定量方法，能够评估滞后链与前导链端粒复制的相对效率，从而提供有关相关机制的信息。在此，我们描述一种用于端粒链特异性分析的简单方法，并讨论其潜在应用。
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