Universiti Tunku Abdul Rahman, Kuala Lumpur, Wilayah Persekutuan, Malaysia.
Biochem Genet. 2011 Oct;49(9-10):562-75. doi: 10.1007/s10528-011-9431-y. Epub 2011 Apr 3.
This study describes the organization of the repetitive pattern in the mtDNA control region of Tomistoma schlegelii. Using newly designed primers, we detected length variations of approximately 50-100 bp among individuals, and only one individual showed a heteroplasmic band. Sequencing the region after CSB III revealed two main patterns: a repeat motif and a variable number tandem repeat (VNTR) pattern. The VNTR region, with a core unit of 104 bp, consisting of four motifs and a short AT chain, is implicated in the length variation seen among individuals of Tomistoma. A conserved motif seen in a family unit indicated that the repeat pattern was stably inherited from the maternal parent to all offspring. A combination of VNTR patterns specific to different crocodilians was seen in Tomistoma, and the overall secondary structure was shown to be similar to that in Crocodylus and Gavialis.
本研究描述了白鱀豚 mtDNA 控制区重复模式的组织。使用新设计的引物，我们在个体间检测到大约 50-100bp 的长度变化，只有一个个体显示异质带。CSB III 后对该区域进行测序揭示了两种主要模式：重复基序和可变数串联重复（VNTR）模式。VNTR 区域，核心单元为 104bp，由四个基序和一个短的 AT 链组成，与个体间的长度变化有关。在一个家族单元中看到的保守基序表明，重复模式从母系稳定遗传给所有后代。白鱀豚中存在与不同鳄类特异性的 VNTR 模式组合，并且整体二级结构与鳄鱼和长吻鳄相似。
