Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 53300, Kuala Lumpur, Malaysia.
Biochem Genet. 2013 Apr;51(3-4):275-95. doi: 10.1007/s10528-012-9562-9. Epub 2013 Jan 17.
The genetic diversity of the endangered crocodile Tomistoma schlegelii was characterized using the protein coding ND 6-tRNA(glu)-cyt b and the cytochrome b-control region (cyt b-CR) markers. Concatenate data revealed six haplotypes with an overall haplotype diversity of 0.769 ± 0.039; nucleotide diversity was 0.00535 ± 0.00172. A nearest-neighbor analysis showed that all individuals clustered with four geographic regions (Sumatra, Peninsular Malaysia, Sarawak, and East Kalimantan) and were genetically differentiated. With the exception of the individuals from haplotype H2, which occurred in both Peninsular Malaysia and Sarawak, all other haplotypes were geographically distinct. The H4 lineage, which was found to be the most divergent, clustered exclusively in the basal clade in all phylogenetic trees, and the haplotype network was unconnected at the 95% reconnection limit, suggesting further investigation to establish its possible status as a distinct evolutionary significant unit or a cryptic species.
采用蛋白质编码 ND6-tRNA(GlU)-cytb 和细胞色素 b 控制区（cytb-CR）标记物，对濒危鳄种暹罗鳄的遗传多样性进行了研究。串联数据显示有 6 种单倍型，总体单倍型多样性为 0.769 ± 0.039；核苷酸多样性为 0.00535 ± 0.00172。最近邻分析表明，所有个体都与四个地理区域（苏门答腊、马来半岛、沙捞越和东加里曼丹）聚类，并存在遗传分化。除了单倍型 H2 的个体同时出现在马来半岛和沙捞越之外，所有其他单倍型都具有地理上的差异。在所有的系统发育树中，发现 H4 谱系是最具分歧的，仅聚集在基部分支中，单倍型网络在 95%的重连限制处没有连接，这表明需要进一步的调查来确定其可能作为一个独特的进化显著单元或隐种的地位。
