Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062 Jilin, China.
Mol Cell Biochem. 2011 Aug;354(1-2):39-46. doi: 10.1007/s11010-011-0803-8. Epub 2011 Apr 3.
Human Fibroblast growth factor 1 (FGF1) has been recognized as a valuable protein drug for the treatment of many diseases because of its multiple functions in regulating a variety of biological processes involved in embryonic development, cell growth and differentiation, morphogenesis, tissue repair, and others. The aim of this study was to develop an FGF1 mammary gland-specific expression vector to produce FGF1 on a large scale from transgenic cows to meet the demand for FGF1 in medical use. In this study, we generated an FGF1 mammary gland-specific expression vector and validated its function in human MCF-7 cells. This vector was shown to successfully express functional FGF1, thus potentially enabling the generation of transgenic cows to be used as mammary gland bioreactors.
人类成纤维细胞生长因子 1（FGF1）因其在调节胚胎发育、细胞生长和分化、形态发生、组织修复等多种生物过程中的多种功能，已被认为是一种有价值的治疗多种疾病的蛋白质药物。本研究旨在开发一种 FGF1 乳腺特异性表达载体，以便从转基因奶牛中大规模生产 FGF1，以满足医用 FGF1 的需求。在本研究中，我们构建了 FGF1 乳腺特异性表达载体，并在人 MCF-7 细胞中验证了其功能。该载体能够成功表达功能性 FGF1，因此有望生成可用于乳腺生物反应器的转基因奶牛。
