Commissariat à l'Energie Atomique, Direction des Sciences du Vivant, Institut de Recherches en Technologies et Sciences pour le Vivant, Laboratoire de Physiologie Cellulaire Végétale, Grenoble, France.
Proteomics. 2011 May;11(9):1650-63. doi: 10.1002/pmic.201000645. Epub 2011 Apr 4.
Monitoring molecular dynamics of an organism upon stress is probably the best approach to decipher physiological mechanisms involved in the stress response. Quantitative analysis of proteins and metabolites is able to provide accurate information about molecular changes allowing the establishment of a range of more or less specific mechanisms, leading to the identification of major players in the considered pathways. Such tools have been successfully used to analyze the plant response to cadmium (Cd), a major pollutant capable of causing severe health issues as it accumulates in the food chain. We present a summary of proteomics and metabolomics works that contributed to a better understanding of the molecular aspects involved in the plant response to Cd. This work allowed us to provide a finer picture of general signaling, regulatory and metabolic pathways that appeared to be affected upon Cd stress. In particular, we conclude on the advantage of employing different approaches of global proteome- and metabolome-wide techniques, combined with more targeted analysis to answer molecular questions and unravel biological networks. Finally, we propose possible directions and methodologies for future prospectives in this field, as many aspects of the plant-Cd interaction remain to be discovered.
监测生物体在应激时的分子动态可能是破译应激反应中涉及的生理机制的最佳方法。对蛋白质和代谢物进行定量分析能够提供关于分子变化的准确信息，从而确定一系列或多或少特定的机制，进而鉴定出所考虑途径中的主要参与者。这些工具已成功用于分析植物对镉（Cd）的反应，镉是一种主要的污染物，它在食物链中积累，会对健康造成严重影响。我们总结了参与植物对 Cd 反应的分子方面的蛋白质组学和代谢组学工作。这项工作使我们能够更详细地了解一般信号转导、调控和代谢途径，这些途径在 Cd 胁迫下似乎受到了影响。特别是，我们得出了这样的结论：采用不同的方法，包括全局蛋白质组学和代谢组学技术，结合更有针对性的分析，以回答分子问题并揭示生物网络，这是有优势的。最后，我们针对该领域的未来展望提出了可能的方向和方法，因为植物与 Cd 的相互作用还有许多方面有待发现。
