Munyai Rabelani, Raletsena Maropeng Vellry, Modise David Mxolisi
Department of Agriculture and Animal Health, Florida Science Campus, University of South Africa, Roodepoort 1709, South Africa.
Faculty of Natural and Agricultural Sciences, Potchefstroom Campus, North West University, Private Bag X6001, Potchefstroom 2520, South Africa.
Metabolites. 2022 Mar 2;12(3):221. doi: 10.3390/metabo12030221.
In water-scarce areas, the reuse of (un)treated acid mine drainage (AMD) water for crop irrigation has become a requirement, but it also carries a wide range of contaminants that can elicit the synthesis of diverse metabolites necessary for the survival of the plants. There is still a paucity of studies on the impact of quicklime treated-AMD water on the metabolite synthesis of potatoes. This study examined the effect of the irrigation of two potato cultivars (Marykies and Royal cultivars) with quicklime-treated AMD water on their metabolite profiles. A greenhouse study was conducted with five experimental treatments with different solution ratios, replicated three times in a completely randomized design. A total of 40 and 36 metabolites from Marykies and Royal cultivars which include amino acids, organic acids, and aromatic amines were identified, respectively. The results revealed elevation in the abundance of metabolites under the irrigation with treated AMD water for both cultivars with subtle variations. This will provide information on the primary metabolite shifst in potato that enhance their survival and growth under AMD conditions. However, more specific data on toxicity due to AMD irrigation would be required for a refined risk assessment.
在水资源匮乏地区，将（未）处理的酸性矿山排水（AMD）水再利用于作物灌溉已成为一种必要手段，但这种水含有多种污染物，可能会引发植物生存所需的多种代谢物的合成。关于生石灰处理后的AMD水对马铃薯代谢物合成的影响，目前的研究仍然较少。本研究考察了用生石灰处理后的AMD水灌溉两个马铃薯品种（玛丽基斯和皇家品种）对其代谢物谱的影响。进行了一项温室研究，设置了五种不同溶液比例的实验处理，以完全随机设计重复三次。玛丽基斯和皇家品种分别鉴定出了总共40种和36种代谢物，包括氨基酸、有机酸和芳香胺。结果显示，两个品种在用处理后的AMD水灌溉下，代谢物丰度均有所升高，但存在细微差异。这将提供关于马铃薯主要代谢物变化的信息，有助于其在AMD条件下的生存和生长。然而，为了进行更精确的风险评估，还需要更多关于AMD灌溉毒性的具体数据。
