Wild P, Schraner E M
Veterinär-Anatomisches Institut, Universität Zürich.
Histochemistry. 1990;94(4):409-14. doi: 10.1007/BF00266448.
Activities of Ca2(+)-dependent ATPase, Mg2(+)-dependent ATPase, Na(+)-K(+)-dependent ATP-ase, alkaline phosphatase, and 5'-nucleotidase were demonstrated after incubation of 40-microns vibratome sections of bovine parathyroids and subsequent visualization by electron microscopy. Prior to sectioning, parathyroid tissue was fixed with 1% glutaraldehyde for localization of alkaline phosphatase, and with 2% formaldehyde and 1% glutaraldehyde for demonstration activities of ATPases and 5'-nucleotidase. The activities of the five enzymes were found at the apicolateral domain of the plasma membrane in parathyroid cells, i.e. at the site parathyroid cells face neighbouring parenchymal cells. Ca2(+)-ATPase activity was also seen on mitochondria, Golgi complex and RER. The presence of these plasma membrane associated enzymes at the apicolateral domain only indicate polarity in parathyroid cells. It further suggests that many processes including transmembrane transport take place at the apicolateral domain, the site of parathyroid cells opposing blood capillaries.
在对牛甲状旁腺40微米厚的振动切片进行孵育，并随后通过电子显微镜观察后，证实了钙离子依赖性ATP酶、镁离子依赖性ATP酶、钠钾依赖性ATP酶、碱性磷酸酶和5'-核苷酸酶的活性。在切片之前，甲状旁腺组织用1%戊二醛固定以定位碱性磷酸酶，并用2%甲醛和1%戊二醛来显示ATP酶和5'-核苷酸酶的活性。在甲状旁腺细胞的质膜顶端外侧区域，即甲状旁腺细胞面对相邻实质细胞的部位，发现了这五种酶的活性。在线粒体、高尔基体复合体和粗面内质网上也可见钙离子ATP酶活性。这些与质膜相关的酶仅在顶端外侧区域存在，表明甲状旁腺细胞具有极性。这进一步表明，包括跨膜运输在内的许多过程发生在顶端外侧区域，即甲状旁腺细胞与毛细血管相对的部位。
