Yao Meiling, Zhang Xingxiao, Gao Jing, Chai Tongjie, Miao Zengmin, Ma Weiming, Qin Mei, Li Qinglei, Li Xiaoxia, Liu Jingbo, Zhang Hongshuang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Research Center for Animal Disease Control Engineering of Shandong Province, Taian, Shandong, China.
Berl Munch Tierarztl Wochenschr. 2011 Mar-Apr;124(3-4):136-41.
To better understand the transmission route of H9N2 avian influenza virus (AIV), two duplicate trials were conducted to observe the process of aerosol infection and direct contact in specific pathogen free chickens. Fifteen chickens (G1) were inoculated with H9N2 AIV and housed together with another 15 chickens (G2) in the same positive-negative-pressure isolator (A). Fifteen chickens (G3) were bred in another isolator (B) which was connected with A so that air could flow unidirectionally from A to B. Air, oropharyngeal and cloacal swabs, and blood samples were collected for the detection of aerosolized virus, virus shedding, and seroconversion. AIV aerosols were initially detected at day 2-3 post inoculation (dpi), reaching peak concentrations at 7 dpi. Virus shedding was detected in all chickens of G2, but only in a part in G3 (T1: 87%, T2: 80%). Antibodies were initially detected at 4-5 dpi, peaking at 14-21 dpi. The results showed that H9N2 AIV could be transmitted by both aerosol exposure and direct contact.
为了更好地了解H9N2禽流感病毒（AIV）的传播途径，进行了两项重复试验，以观察特定病原体-free鸡的气溶胶感染和直接接触过程。15只鸡（G1）接种H9N2 AIV，并与另外15只鸡（G2）一起饲养在同一个正负压隔离器（A）中。15只鸡（G3）饲养在另一个与A相连的隔离器（B）中，以便空气能从A单向流向B。采集空气、口咽和泄殖腔拭子以及血液样本，用于检测气溶胶化病毒、病毒排泄和血清转化。接种后第2 - 3天（dpi）首次检测到AIV气溶胶，在7 dpi时达到峰值浓度。在G2组的所有鸡中均检测到病毒排泄，但在G3组中只有部分鸡检测到（T1：87%，T2：80%）。抗体在4 - 5 dpi时首次检测到，在14 - 21 dpi时达到峰值。结果表明，H9N2 AIV可通过气溶胶暴露和直接接触传播。
