Breuer Julia, Müller Uwe, Locher Lena, Spallek Alice, Recknagel Stephan, Uhlig Albrecht, Schusser Gerald Fritz
Medizinische Tierklinik, Veterinärmedizinische Fakultät, Universität Leipzig, Leipzig, Germany.
Berl Munch Tierarztl Wochenschr. 2011 Mar-Apr;124(3-4):154-60.
Proinflammatoric cytokines are released extracellularly during necrosis. These lead to inflammation and destruction of surrounding tissues. The aim of this study was to compare the number of viable, apoptotic and necrotic cells in the bronchoalveolar lavage fluid (BALF) of normal horses and horses with recurrent airway obstruction (RAO) and to determine if fluorescence microscopy is a reliable method for this examination. A group of six normal horses and a group of ten horses with RAO were examined. Samples were assessed using annexin-V and propidium iodide immunofluorescence assay and examined by fluorescence microscopy (16 horses) and flow cytometry (nine of 16 horses). We found no significant differences in percentages of apoptotic and viable cells between both groups. The number of necrotic cells was significantly increased in horses with RAO counted by fluorescence microscopy. Cells with high granularity and macrophages had a significantly higher percentage of necrotic cells than lymphocytes. There was a good agreement between both methods. No significant differences were detected. The correlation between both methods is significant. Higher amounts of necrotic cells in the bronchial lumina of horses with RAO could be a reason for tissue damage and continuous lung tissue inflammation. Fluorescence microscopy was applicable for examination of BALF. Therapy should be aimed at the reduction of necrotic cells in the bronchial lumina. Further studies are required to find ways to reduce inflammatory cell infiltration and necrosis in bronchial lumina.
促炎细胞因子在坏死过程中释放到细胞外。这些会导致周围组织的炎症和破坏。本研究的目的是比较正常马匹和患有复发性气道阻塞（RAO）的马匹支气管肺泡灌洗液（BALF）中活细胞、凋亡细胞和坏死细胞的数量，并确定荧光显微镜检查是否是一种可靠的检测方法。对一组6匹正常马匹和一组10匹患有RAO的马匹进行了检查。使用膜联蛋白-V和碘化丙啶免疫荧光测定法评估样本，并通过荧光显微镜检查（16匹马）和流式细胞术（16匹马中的9匹）进行检测。我们发现两组之间凋亡细胞和活细胞的百分比没有显著差异。通过荧光显微镜计数，患有RAO的马匹中坏死细胞的数量显著增加。高颗粒度细胞和巨噬细胞的坏死细胞百分比明显高于淋巴细胞。两种方法之间具有良好的一致性。未检测到显著差异。两种方法之间的相关性显著。患有RAO的马匹支气管腔中坏死细胞数量增加可能是组织损伤和肺组织持续炎症的原因。荧光显微镜适用于BALF的检查。治疗应旨在减少支气管腔中的坏死细胞。需要进一步研究以找到减少支气管腔中炎性细胞浸润和坏死的方法。
