The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
Phytochem Anal. 2011 Nov-Dec;22(6):475-83. doi: 10.1002/pca.1303. Epub 2011 Apr 4.
Spirodela polyrrhiza (L.) Schleid. is a traditional Chinese herbal medicine for the treatment of influenza. Despite its wide use in Chinese medicine, no report on quality control of this herb is available so far.
To establish qualitative and quantitative analytical methods by high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) for the quality control of S. polyrrhiza.
The methanol extract of S. polyrrhiza was analysed by HPLC/ESI-MS(n). Flavonoids were identified by comparing with reference standards or according to their MS(n) (n = 2-4) fragmentation behaviours. Based on LC/MS data, a standardised HPLC fingerprint was established by analysing 15 batches of commercial herbal samples. Furthermore, quantitative analysis was conducted by determining five major flavonoids, namely luteolin 8-C-glucoside, apigenin 8-C-glucoside, luteolin 7-O-glucoside, apigenin 7-O-glucoside and luteolin.
A total of 18 flavonoids were identified by LC/MS, and 14 of them were reported from this herb for the first time. The HPLC fingerprints contained 10 common peaks, and could differentiate good quality batches from counterfeits. The total contents of five major flavonoids in S. polyrrhiza varied significantly from 4.28 to 19.87 mg/g.
Qualitative LC/MS and quantitative HPLC analytical methods were established for the comprehensive quality control of S. polyrrhiza.
螺旋藻（L.）施莱德是一种传统的中药，用于治疗流感。尽管它在中国医学中被广泛应用，但迄今为止尚无关于该草药质量控制的报告。
建立高效液相色谱法（HPLC）与质谱（MS）联用的定性和定量分析方法，用于螺旋藻的质量控制。
采用 HPLC/ESI-MS（n）分析螺旋藻的甲醇提取物。通过与对照品比较或根据其 MS（n）（n=2-4）碎片行为来鉴定黄酮类化合物。基于 LC/MS 数据，通过分析 15 批商业草药样品建立了标准化的 HPLC 指纹图谱。此外，通过测定 5 种主要黄酮类化合物（即木犀草素 8-C-葡萄糖苷、芹菜素 8-C-葡萄糖苷、木犀草素 7-O-葡萄糖苷、芹菜素 7-O-葡萄糖苷和木犀草素）进行定量分析。
通过 LC/MS 鉴定出 18 种黄酮类化合物，其中 14 种是首次从该植物中报道的。HPLC 指纹图谱包含 10 个共有峰，可以区分优质批次和假冒产品。螺旋藻中 5 种主要黄酮类化合物的总含量差异显著，从 4.28 到 19.87mg/g 不等。
建立了定性 LC/MS 和定量 HPLC 分析方法，用于全面控制螺旋藻的质量。
