Developmental Biology Unit, Institute of Biology I, Faculty of Biology, University of Freiburg, Freiburg, Germany.
Dev Dyn. 2011 Jun;240(6):1578-85. doi: 10.1002/dvdy.22632. Epub 2011 Apr 4.
Ephrins and their tyrosine kinase receptors (Ephs) are a highly conserved family of signaling proteins with various functions during embryonic development. Among others, Eph/ephrin signaling is involved in regulating axon guidance, cell migration, and tissue border formation through inducing modifications of the actin cytoskeleton and cell adhesion. During development ephrins and Ephs are expressed in spatially and temporarily regulated patterns in a wide range of tissues. Here, we analyzed the expression of seven members of the Eph and four member of the ephrin family during early stages of mouse inner ear development by whole-mount in situ hybridization. We detected expressions of EphA2, EphA4, EphA7, EphB1, ephrinA4, and ephrinA5 in and around the forming otic placode between embryonic day (E) 8.5 and E10, and report their detailed expression patterns. Our results reveal dynamic expression of several members of the ephrin/Eph family consistent with functions in otic placode development, invagination and neuroblast delamination.
Eph 蛋白及其酪氨酸激酶受体（Ephs）是一类高度保守的信号蛋白家族，在胚胎发育过程中具有多种功能。其中，Eph/ephrin 信号通路通过诱导肌动蛋白细胞骨架和细胞黏附的改变，参与调节轴突导向、细胞迁移和组织边界形成。在发育过程中，ephrin 和 Eph 以广泛的时空调节模式在多种组织中表达。在这里，我们通过整体原位杂交分析了 Eph 家族的 7 个成员和 ephrin 家族的 4 个成员在小鼠内耳发育早期阶段的表达。我们在胚胎期 8.5 天至 10 天（E）之间的形成的听基板中检测到 EphA2、EphA4、EphA7、EphB1、ephrinA4 和 ephrinA5 的表达，并报告了它们的详细表达模式。我们的结果揭示了 Eph 家族的几个成员的动态表达，与听基板发育、内陷和神经母细胞分层的功能一致。
