Analytical Chemistry Department, Faculty of Chemistry, University of Tabriz, Iran.
Luminescence. 2011 Nov-Dec;26(6):622-8. doi: 10.1002/bio.1285. Epub 2011 Apr 4.
A highly selective and simple chemiluminescence (CL) method for determination of penicillin G potassium (PGK) was developed. In the proposed method, CL was elicited from PGK upon its oxidation with H(2)O(2). The light emission was enhanced in the presence of N-cetyl-N,N,N-trimethylammonium bromide (CTMAB). An experimental design, central composite design (CCD), was used to realize the optimized variables, including pH, surfactant (CTMAB) and H(2)O(2) concentrations. Under optimum condition, the calibration graph was linear in the range 3.3 × 10(-3) -3.3 × 10(-1) mmol/L, with a detection limit of 8.8 × 10(-4) mmol/L for PGK. The precision was calculated by analysing samples containing 1.6 × 10(-1) mmol/L PGK (n = 5) and the relative standard deviation (RSD) was 1.40%. The utility of this method was demonstrated by determining PGK in pharmaceutical formulations for injection. The proposed method was validated by a reference method.
一种高选择性和简单的化学发光（CL）方法被开发出来，用于测定青霉素 G 钾（PGK）。在提出的方法中，PGK 被 H（2）O（2）氧化后引发 CL。在存在 N-十六烷基-N，N，N-三甲基溴化铵（CTMAB）的情况下，发光增强。实验设计，中心复合设计（CCD）用于实现最佳变量，包括 pH 值、表面活性剂（CTMAB）和 H（2）O（2）浓度。在最佳条件下，校准曲线在 3.3×10（-3）-3.3×10（-1）mmol/L 范围内呈线性，PGK 的检测限为 8.8×10（-4）mmol/L。通过分析含有 1.6×10（-1）mmol/L PGK 的样品（n=5）来计算精密度，相对标准偏差（RSD）为 1.40%。该方法通过测定注射用药物制剂中的 PGK 得到了验证。该方法通过参考方法进行了验证。
