Ma Hong-Yan
College of Chemistry and Chemical Engineering, Provincial Key Laboratory of Chemical Reaction Engineering, Yanan University, Yanan 716000, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Aug;25(8):1210-2.
A new flow injection electrogenerated chemiluminescence (ECL) method for the determination of genyamicin sulfate was developed. The method is based on the sensitizing effect of genyamicin sulfate on the weak ECL signal of electrochemical oxidation of luminol on the platinum flake electrode in the medium of Na2B4O7. The experimental conditions that influence the ECL intensity were investigated and optimized. Under the optimum conditions, the ECL response is in the range of 1.2 x 10(-9) - 4.0 x 10(-6) g x mL(-1) genyamicin sulfate with a detection limit of 8.0 x 10(-10) g x mL(-1). The relative standard deviation is 2.0%. This method was used to determine genyamicin sulfate in injection samples with satisfactory results.
建立了一种测定硫酸庆大霉素的流动注射电化学发光（ECL）新方法。该方法基于在硼酸钠介质中，硫酸庆大霉素对鲁米诺在铂片电极上电化学氧化产生的微弱ECL信号的增敏作用。研究并优化了影响ECL强度的实验条件。在最佳条件下，硫酸庆大霉素的ECL响应范围为1.2×10⁻⁹ - 4.0×10⁻⁶ g·mL⁻¹，检测限为8.0×10⁻¹⁰ g·mL⁻¹。相对标准偏差为2.0%。该方法用于测定注射剂样品中的硫酸庆大霉素，结果令人满意。
