School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Luminescence. 2011 Nov-Dec;26(6):629-33. doi: 10.1002/bio.1286. Epub 2011 Apr 4.
Based on the inhibition effect of transferrin (Tf) on the reaction of the luminol-hydrogen peroxide (H(2)O(2)) chemiluminescence (CL) system, catalysed by meso-tetra-(3-methoxyl-4-hydroxyl) phenyl manganese porphyrin (MnP) as a mimetic enzyme of peroxides, a sensitive flow-injection CL method has been developed for the determination of Tf in an alkaline medium. The CL reaction was carefully investigated by examining the variations of reaction conditions. Under optimum conditions, the linear range for the determination of transferrin was 0.04-20.0 μg/mL and the detection limit was 1.62 ng/mL. This proposed method was sensitive, convenient and simple, and has been successfully applied to the determination of transferrin in a serum sample.
基于转铁蛋白（Tf）对模拟过氧化物酶 meso-四-(3-甲氧基-4-羟基)苯基锰卟啉（MnP）催化的鲁米诺-过氧化氢（H2O2）化学发光（CL）体系反应的抑制作用，建立了一种在碱性介质中测定 Tf 的灵敏流动注射 CL 法。通过考察反应条件的变化，仔细研究了 CL 反应。在最佳条件下，测定转铁蛋白的线性范围为 0.04-20.0 μg/mL，检测限为 1.62 ng/mL。该方法灵敏、简便、快速，已成功应用于血清样品中转铁蛋白的测定。
