主动脉瓣关闭不全大鼠心房利钠肽信使核糖核酸与肽

Atrial natriuretic peptide messenger RNA and peptide in rats with aortic valve insufficiency.

作者信息

Morita H, Tanaka I, Oda T, Ichiyama A, Yamazaki T, Uematsu T, Nakashima M, Yoshimi T

机构信息

Second Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.

出版信息

Peptides. 1990 Jul-Aug;11(4):843-7. doi: 10.1016/0196-9781(90)90202-g.

DOI:10.1016/0196-9781(90)90202-g

PMID:2146597

Abstract

To investigate ANP gene expression in diseased hearts of animals without genetic defects, immunoreactive ANP (IR-ANP) and ANP mRNA were measured in a rat with aortic valve insufficiency (AI), which was produced by puncturing one of the aortic valve leaflets with a plastic rod. Plasma IR-ANP concentration was higher in AI rats than in sham rats (p less than 0.05). Decreased atrial concentration of IR-ANP (p less than 0.05) and unchanged atrial ANP mRNA concentration were shown in AI rats. The ventricular concentrations of IR-ANP and ANP mRNA in AI rats were 5.4 and 2.4 times higher than those in sham rats (p less than 0.05, respectively). These results demonstrate that gene expression of ventricular ANP is markedly increased in AI rats while that of atrial ANP is not changed.

摘要

为研究无基因缺陷的患病动物心脏中的心房钠尿肽（ANP）基因表达，我们检测了一只通过用塑料棒穿刺主动脉瓣小叶之一而造成主动脉瓣关闭不全（AI）的大鼠的免疫反应性ANP（IR-ANP）和ANP mRNA。AI大鼠的血浆IR-ANP浓度高于假手术大鼠（p<0.05）。AI大鼠的心房IR-ANP浓度降低（p<0.05），而心房ANP mRNA浓度未变。AI大鼠心室中的IR-ANP和ANP mRNA浓度分别比假手术大鼠高5.4倍和2.4倍（p均<0.05）。这些结果表明，AI大鼠心室ANP的基因表达显著增加，而心房ANP的基因表达未改变。