Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia.
Pol J Microbiol. 2010;59(4):233-9.
Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA.
生物膜形成的多药耐药葡萄球菌是眼部感染传播的主要储库。它们从患有结膜炎的眼部患者中分离出来。在这项研究中，我们分析了从临床眼部患者中分离出的葡萄球菌的生物膜形成能力、抗生素耐药谱，以及它们与其他社区耐甲氧西林金黄色葡萄球菌（MRSA）的系统发育关系。从临床标本中分离出的 60 株葡萄球菌用于评估其形成生物膜和表达生物膜编码 ica 基因的能力。其中 93%是粘液产生菌，87%是粘液阳性菌。金黄色葡萄球菌和表皮葡萄球菌是从眼部患者中分离出的主要菌株。这些菌株的生物膜形成能力也存在差异。用青霉素 G、环丙沙星、氧氟沙星、甲氧西林、阿米卡星和庆大霉素对这些菌株进行的抗生素敏感性测试表明，它们对一种以上的抗生素有耐药性。通过聚合酶链反应获得的强生物膜产生的金黄色葡萄球菌菌株的 ica 基因扩增子被测序，并根据系统发育树分析了它们与社区获得性 MRSA 的密切遗传关系。我们的研究结果表明，它们在遗传上与其他社区获得性 MRSA 密切相关。
