Avakian A P, Kleven S H
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30606.
Vet Microbiol. 1990 Aug;24(2):155-69. doi: 10.1016/0378-1135(90)90063-2.
The humoral immune response over time of White Leghorn chickens experimentally infected with Mycoplasma gallisepticum or M. synoviae by an aerosol inoculation or a contact exposure were compared by immunoblotting. The response of chickens infected with M. gallisepticum were similar with respect to proteins recognized and intensity of response, regardless of mode of infection. On the other hand, chickens infected by aerosolization of M. synoviae responded to more proteins and with greater intensity than did M. synoviae contact-exposed birds. Chickens infected with M. gallisepticum responded with antibodies to over 20 proteins, while chickens infected with M. synoviae responded with antibodies to 12 proteins. Field sera from chickens naturally infected on commercial poultry farms with M. gallisepticum or M. synoviae were analyzed by immunoblotting and were found to react with a number of mycoplasma proteins. However, no correlation was seen when comparing intensity of immunoblot staining and hemagglutination-inhibition titer of the field sera. The experimental antisera were used to identify species-specific proteins of M. gallisepticum and M. synoviae. Six immunogenic species-specific proteins of M. gallisepticum with relative molecular masses of 82 (p82), 65-63 (p64), 56 (p56), 35 (p35), 26 (p26), and 24 (p24) kilodaltons (kDa) were identified. Two species-specific proteins of M. synoviae with relative molecular masses of 53 (p53) and 22 (p22) kDa were identified. Additionally, a highly immunogenic 41 (p41) kDa protein of M. synoviae was identified. Species-specific proteins identified in these mycoplasmas and the 41 kDa protein of M. synoviae were purified by preparative SDS-PAGE in amounts sufficient for further characterization and for use in serodiagnostic tests.
通过免疫印迹法比较了经气溶胶接种或接触暴露实验性感染鸡毒支原体或滑液支原体的白来航鸡随时间的体液免疫反应。感染鸡毒支原体的鸡，无论感染方式如何，在识别的蛋白质和反应强度方面反应相似。另一方面，经气溶胶感染滑液支原体的鸡比经接触暴露感染滑液支原体的鸡对更多蛋白质产生反应且反应强度更大。感染鸡毒支原体的鸡产生针对20多种蛋白质的抗体，而感染滑液支原体的鸡产生针对12种蛋白质的抗体。对商业家禽场自然感染鸡毒支原体或滑液支原体的鸡的现场血清进行免疫印迹分析，发现其与多种支原体蛋白发生反应。然而，比较现场血清的免疫印迹染色强度和血凝抑制效价时未发现相关性。实验抗血清用于鉴定鸡毒支原体和滑液支原体的种特异性蛋白。鉴定出鸡毒支原体的六种免疫原性种特异性蛋白，相对分子质量分别为82（p82）、65 - 63（p64）、56（p56）、35（p35）、26（p26）和24（p24）千道尔顿（kDa）。鉴定出滑液支原体的两种种特异性蛋白，相对分子质量分别为53（p53）和22（p22）kDa。此外，还鉴定出滑液支原体一种高度免疫原性的41（p41）kDa蛋白。通过制备性SDS - PAGE纯化这些支原体中鉴定出的种特异性蛋白以及滑液支原体的41 kDa蛋白，纯化量足以进行进一步表征并用于血清诊断试验。
