Avakian A P, Ley D H, McBride M A
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Avian Dis. 1992 Jan-Mar;36(1):69-77.
Two putative variant Mycoplasma gallisepticum (MG) strains (M876 and M35), originally isolated from commercial turkeys, were compared with eight well-characterized MG strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE protein profiles indicated that the variant strains were correctly classified as MG based on homologous patterns in species-specific regions of the electrophoretic profiles. However, differences in protein profiles also indicated that variant strains M876 and M35 were different from each other and the other MG strains tested. Immunoblotting was used to assess the humoral immune response of turkeys to infection with the S6 reference strain or M876 variant strain of MG. Immunoblots using antisera to M876 showed that seroconversion to this isolate was slower, and to fewer MG proteins when compared with immunoblots using antisera to S6. Immunoblot analyses further indicated that pooled antisera from turkeys inoculated with either S6 or M876 reacted with each of 10 MG strains tested. However, pooled S6 antisera reacted with greater intensity and with more MG proteins than did pooled M876 antisera. The species-specific immunodominant proteins with the greatest potential for use as antigens in serologic tests appeared to be those of 64 (p64) and 56 (p56) kilodaltons molecular mass.
将最初从商业火鸡中分离出的两株假定的鸡毒支原体（MG）变异株（M876和M35）与八株特征明确的MG菌株通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行比较。SDS-PAGE蛋白图谱表明，基于电泳图谱物种特异性区域的同源模式，变异株被正确归类为MG。然而，蛋白图谱的差异也表明变异株M876和M35彼此不同，且与所测试的其他MG菌株也不同。免疫印迹法用于评估火鸡对MG的S6参考株或M876变异株感染的体液免疫反应。使用抗M876抗血清的免疫印迹显示，与使用抗S6抗血清的免疫印迹相比，对该分离株的血清转化较慢，且与较少的MG蛋白发生反应。免疫印迹分析进一步表明，接种S6或M876的火鸡的混合抗血清与所测试的10株MG菌株中的每一株都发生反应。然而，与混合的M876抗血清相比，混合的S6抗血清反应强度更大，且与更多的MG蛋白发生反应。在血清学检测中最有潜力用作抗原的物种特异性免疫显性蛋白似乎是分子量为64（p64）和56（p56）千道尔顿的那些蛋白。
