Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Jingzhou 434000, China.
Mol Biol Rep. 2011 Oct;38(7):4595-602. doi: 10.1007/s11033-010-0592-2. Epub 2011 Apr 6.
The growth hormone/prolactin family genes are major participants in control of several complex physiologic processes, including growth, reproduction, and metabolism. In this study, total RNA was isolated from the pituitary of the Prenant's schizothoracin (Schizothorax prenanti), and SMART cDNA was synthesized from 100 ng total RNA. The full-length cDNAs of three GH/PRL family genes have been cloned and sequenced from the SMART cDNA library. The growth hormone of S. prenanti (SpGH) cDNA contains 1238 base pairs (bp) and encodes 215 amino acid (aa) residues; the prolactin (SpPRL) cDNA contains 1124 bp and encodes 209 aa residues; the cDNA sequence of somatolactin (SpSL) is 1290 bp in length, and encodes for a peptide of 234 aa residues. Although there is only about 22% aa sequence identity between the three deduced proteins, SpGH, SpPRL and SpSL, overall, the C-terminal region shows a higher identity. Multiple aa sequence alignments indicated the deduced SpGH, SpPRL and SpSL show high identities to that of goldfish and zebrafish, respectively. RT-PCR analysis revealed that the three hormones displayed different tissue distribution patterns. SpPRL transcripts were detected only in the pituitary of the Prenant's schizothoracin. SpSL was more widely distributed than SpPRL, high mRNA levels of SpSL were detected in the pituitary and low mRNA levels of SpSL were found in liver, kidney and heart. SpGH was expressed in nearly all tissues detected with the highest expression level in the pituitary.
生长激素/催乳素家族基因是控制多种复杂生理过程的主要参与者，包括生长、繁殖和代谢。在这项研究中，从中华鲟（Schizothorax prenanti）的垂体中分离出总 RNA，并从 100ng 总 RNA 合成 SMART cDNA。从 SMART cDNA 文库中克隆和测序了三种 GH/PRL 家族基因的全长 cDNA。中华鲟生长激素（SpGH）cDNA 包含 1238 个碱基对（bp），编码 215 个氨基酸（aa）残基；催乳素（SpPRL）cDNA 包含 1124bp，编码 209aa 残基；生长激素（SpSL）cDNA 序列长 1290bp，编码 234aa 肽。尽管三种推导蛋白 SpGH、SpPRL 和 SpSL 之间的 aa 序列同一性约为 22%，但总体而言，C 末端区域具有更高的同一性。多个 aa 序列比对表明，推导的 SpGH、SpPRL 和 SpSL 与金鱼和斑马鱼的同源性较高。RT-PCR 分析表明，这三种激素在组织中的分布模式不同。SpPRL 转录本仅在中华鲟的垂体中检测到。SpSL 的分布比 SpPRL 更广泛，在垂体中检测到 SpSL 的高 mRNA 水平，在肝脏、肾脏和心脏中检测到 SpSL 的低 mRNA 水平。SpGH 在检测到的几乎所有组织中均有表达，在垂体中的表达水平最高。
