Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Mol Biol Rep. 2012 Apr;39(4):4647-53. doi: 10.1007/s11033-011-1256-6. Epub 2011 Oct 11.
Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.
中华鲟（Acipenser sinensis）是一种珍稀濒危物种，也是鲟鱼养殖业的重要资源，但该物种的几个基因已经被鉴定出来。我们在此报告从一条 24 岁的雌性中华鲟产卵后构建的垂体 cDNA 文库，并从该文库中获得了 2025 个 EST。鉴定出 885 个独特序列，这些序列被分为 12 个功能组。超过一半的独特序列（57%）与公共数据库中的注释序列不匹配。进一步鉴定了其中的三个新基因。值得注意的是，鉴定出一个编码 232 个氨基酸的全长生长激素的 cDNA（1143bp）。系统发育分析显示与白鲟生长激素的氨基酸同一性为 97%。RT-PCR 分析表明，生长激素 mRNA 仅在垂体中检测到。生长激素在垂体中的特异性表达表明，该蛋白可能在中华鲟垂体内分泌系统中发挥重要的生理功能。
